| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 639,164 | G→A | 61.9% | P63P (CCG→CCA) | ahpF → | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 639,164 | 0 | G | A | 61.9% | 10.2 / 20.1 | 21 | P63P (CCG→CCA) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (4/4); new base A (4/9); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
TCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTGGGCCACG > W3110S.gb/639103‑639231 | tCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg < 1:1715499/71‑1 (MQ=255) aCTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCa < 1:826893/71‑1 (MQ=255) aCTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCa < 1:690602/71‑1 (MQ=255) gACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAgg < 1:1243046/71‑1 (MQ=255) cAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCtt < 1:905712/50‑1 (MQ=255) aaaGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTc > 1:981858/1‑71 (MQ=255) aaGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGt > 1:1036831/1‑45 (MQ=255) tCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAAc < 1:1440046/71‑1 (MQ=255) tCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAAc < 1:1748183/71‑1 (MQ=255) ccTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAg < 1:841054/71‑1 (MQ=255) ttAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAgggg > 1:404619/1‑71 (MQ=255) aaaGAAGATAACAGCTTGCCGGTGCGTAAGCCATc > 1:827010/1‑35 (MQ=255) aagaagATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCa > 1:1234662/1‑71 (MQ=255) aTAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACg < 1:1224213/67‑1 (MQ=255) ttGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACg < 1:633613/59‑1 (MQ=255) ttGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACg < 1:1719067/59‑1 (MQ=255) ccGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTccc < 1:704920/70‑1 (MQ=255) gCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTg > 1:137578/1‑56 (MQ=255) gCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTgg > 1:797524/1‑71 (MQ=255) aaGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAgg > 1:1613413/1‑56 (MQ=255) gCCGTCCTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTGGGCCACg < 1:448320/71‑1 (MQ=255) | TCGCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTGGGCCACG > W3110S.gb/639103‑639231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |