| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 651,069 | T→C | 45.5% | N4S (AAT→AGT) | citC ← | citrate lyase synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 651,069 | 0 | T | C | 45.5% | ‑0.9 / 27.8 | 22 | N4S (AAT→AGT) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (5/7); new base C (7/3); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
TTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATG > W3110S.gb/650999‑651138 | ttCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCAt > 1:112099/1‑71 (MQ=255) aTTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCACTGCCGAACata < 1:1651816/71‑1 (MQ=255) aTTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCACTGCCGAACat > 1:1480848/1‑70 (MQ=255) gggCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataa > 1:53143/1‑69 (MQ=255) cGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaa > 1:1268708/1‑70 (MQ=255) ttttttATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataat < 1:647054/54‑1 (MQ=255) ttttttATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataat < 1:1606677/54‑1 (MQ=255) ttttATTTTCTGAACGTTTTACGCGGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTg > 1:1066608/1‑71 (MQ=255) ttttATTTTCTGAACGTTTTACGCGGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTg > 1:1355677/1‑71 (MQ=255) ttCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttg > 1:1529855/1‑71 (MQ=255) tCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttg < 1:1014173/70‑1 (MQ=255) aaCGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATc > 1:17704/1‑71 (MQ=255) aaCGTTTTACGCGGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATc > 1:1396091/1‑71 (MQ=255) aCGTTTTACGCGGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATc > 1:123680/1‑70 (MQ=255) aCGTTTTACGCGGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCg < 1:641924/71‑1 (MQ=255) cgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACg < 1:1119521/71‑1 (MQ=255) gTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGc < 1:573676/64‑1 (MQ=255) tGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTg < 1:1722206/44‑1 (MQ=255) gAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAtgt > 1:1633545/1‑71 (MQ=255) aaaaTATCATTGCCGAACATAATAAATAGTATCCTg < 1:1663121/36‑1 (MQ=255) aaTATCACTGCCGAACATAATAAATAGTATCCTGAAg > 1:782210/1‑37 (MQ=255) atatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTa > 1:474275/1‑49 (MQ=255) aTTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATg < 1:962338/71‑1 (MQ=255) | TTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATG > W3110S.gb/650999‑651138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |