Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 708,650 C→T 26.3% intergenic (+471/‑106) glnS → / → ybfM glutamyl‑tRNA synthetase/predicted outer membrane porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb708,6500CT26.3% 28.5 / 9.0 19intergenic (+471/‑106)glnS/ybfMglutamyl‑tRNA synthetase/predicted outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (8/6);  new base T (3/2);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

GTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTT  >  W3110S.gb/708595‑708705
                                                       |                                                       
gTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAg                                          >  1:1756961/1‑71 (MQ=255)
   cTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTTTGTTCCTGTAGTCAGc                                         >  1:796647/1‑69 (MQ=255)
   cTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTg                                                       >  1:1168437/1‑55 (MQ=255)
        aTTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACtt                                  <  1:576579/71‑1 (MQ=255)
          tCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACtttt                                >  1:1013967/1‑71 (MQ=255)
           cTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTTTg                                                       >  1:1181286/1‑47 (MQ=255)
           cTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACtttt                                <  1:216480/70‑1 (MQ=255)
              gTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTtctc                             >  1:241384/1‑70 (MQ=255)
                       agagATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACtttt                                >  1:1441658/1‑58 (MQ=255)
                       agagATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACtt                   >  1:956332/1‑71 (MQ=255)
                       agagATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACtt                   >  1:343553/1‑71 (MQ=255)
                           aTTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGc                                         <  1:977833/45‑1 (MQ=255)
                           aTTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGc                                         <  1:1278468/45‑1 (MQ=255)
                           aTTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGc                                         <  1:1069511/45‑1 (MQ=255)
                                  aTTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTtctc                             <  1:845595/50‑1 (MQ=255)
                                    tcgcgtcgcgAAAAATAGTTTGTTCCTGTAGTCAGCga                                       <  1:401575/38‑1 (MQ=255)
                                    tcgcgtcgcgAAAAATAGTTTGTTCCTGTAGTCAGCga                                       <  1:54354/38‑1 (MQ=255)
                                    tcgcgtcgcgAAAAATAGTTTGTTCCTGTAGTCAGCGAGACtttt                                >  1:212548/1‑45 (MQ=255)
                                                aaaTAGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAatttttattttt  >  1:1434935/1‑63 (MQ=255)
                                                       |                                                       
GTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTT  >  W3110S.gb/708595‑708705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑