| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 833,307 | G→T | 57.6% | T22T (ACC→ACA) | ybiA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 833,307 | 0 | G | T | 57.6% | ‑3.5 / 37.1 | 26 | T22T (ACC→ACA) | ybiA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (5/6); new base T (9/6); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
GGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATT > W3110S.gb/833239‑833373 | ggCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGt > 1:528074/1‑71 (MQ=255)ggCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGt < 1:672916/71‑1 (MQ=255) tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat < 1:1043117/71‑1 (MQ=255) tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat > 1:1639737/1‑71 (MQ=255) tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat > 1:585896/1‑71 (MQ=255) cTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg > 1:634991/1‑71 (MQ=255) ttGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGgt > 1:790506/1‑71 (MQ=255) ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTc > 1:528461/1‑68 (MQ=255) acAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTa > 1:825377/2‑48 (MQ=255) cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCAt < 1:8253/71‑1 (MQ=255) taaGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGaaa < 1:758027/42‑1 (MQ=255) aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGttt < 1:402023/71‑2 (MQ=255) aGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGc > 1:137727/1‑55 (MQ=255) aGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATc < 1:251934/70‑1 (MQ=255) gAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCt > 1:761935/1‑71 (MQ=255) gAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCt < 1:669726/71‑1 (MQ=255) aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAAttt > 1:972719/1‑36 (MQ=255) aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAAttt > 1:329150/1‑36 (MQ=255) ccGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCg < 1:156116/71‑1 (MQ=255) tAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCAt < 1:15913/44‑1 (MQ=255) tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgt < 1:117947/46‑1 (MQ=255) aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTg > 1:1115466/1‑48 (MQ=255) aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCa < 1:29324/71‑1 (MQ=255) tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCtt < 1:832093/54‑1 (MQ=255) gCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCAtt > 1:1631100/1‑70 (MQ=255) gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTtg > 1:753136/1‑54 (MQ=255) | GGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATT > W3110S.gb/833239‑833373 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |