| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 861,111 | G→A | 46.9% | L640L (CTG→TTG) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 861,111 | 0 | G | A | 46.9% | 2.4 / 41.4 | 32 | L640L (CTG→TTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (8/9); new base A (8/7); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01 | |||||||||||
TGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACT > W3110S.gb/861043‑861179 | tgaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAAtt > 1:482732/1‑71 (MQ=255) cGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt > 1:1646436/1‑71 (MQ=255) aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTc > 1:1086887/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:1128239/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:794715/71‑1 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGAt > 1:379092/1‑70 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGAt > 1:1541288/1‑70 (MQ=255) gccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAgg < 1:241838/59‑1 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1735579/1‑71 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGa > 1:1153114/1‑50 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAg > 1:576372/1‑71 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGCTACTGTTTCAATTCGTCGa > 1:1700995/1‑50 (MQ=38) aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1738525/1‑49 (MQ=255) aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAAtt < 1:216067/43‑1 (MQ=255) gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcag > 1:1185233/1‑70 (MQ=255) aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg > 1:1156623/1‑71 (MQ=255) aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTAt < 1:559479/71‑1 (MQ=255) cGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCg < 1:943571/71‑1 (MQ=255) gTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:612948/65‑1 (MQ=255) gTAGCCCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:1327454/65‑1 (MQ=255) tAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:121567/36‑1 (MQ=255) tAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa > 1:74334/1‑70 (MQ=255) ttATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:798696/38‑1 (MQ=255) tATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcg > 1:1196578/1‑71 (MQ=255) aTGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcg < 1:686414/70‑1 (MQ=255) aTGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa > 1:43238/1‑59 (MQ=255) gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag < 1:893660/48‑1 (MQ=255) gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgt < 1:1242903/68‑1 (MQ=255) gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgt < 1:734737/68‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:20794/37‑1 (MQ=255) tACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCca < 1:1672923/43‑1 (MQ=255) aGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACt < 1:358786/70‑1 (MQ=255) | TGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACT > W3110S.gb/861043‑861179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |