| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 891,153 | C→T | 61.8% | R8H (CGT→CAT) | grxA ← | glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 891,153 | 0 | C | T | 61.8% | 22.0 / 35.1 | 34 | R8H (CGT→CAT) | grxA | glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
| Reads supporting (aligned to +/- strand): ref base C (6/7); new base T (11/10); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTA > W3110S.gb/891085‑891217 | aaTCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGa > 1:17050/1‑71 (MQ=255)aaTCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGa > 1:1672194/1‑71 (MQ=255) cgTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAAt < 1:959660/71‑1 (MQ=255) gTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAAt < 1:957667/70‑1 (MQ=255) cATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACg > 1:296779/1‑71 (MQ=255) cATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACg > 1:1338946/1‑71 (MQ=255) cATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACg > 1:1595852/1‑71 (MQ=255) aTTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACgg < 1:519130/71‑1 (MQ=255) aTTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACgg < 1:274370/71‑1 (MQ=255) gCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGtt < 1:143393/70‑1 (MQ=255) tCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACg < 1:1138731/65‑1 (MQ=255) tttCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGt < 1:1207713/63‑1 (MQ=255) ttCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGttt < 1:864932/64‑1 (MQ=255) ttCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCattat > 1:1133398/1‑71 (MQ=255) aTCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTg < 1:1372708/54‑1 (MQ=255) aTCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTg < 1:756112/54‑1 (MQ=255) cTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATaa > 1:1385838/1‑45 (MQ=255) ttttGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTAttt < 1:851540/59‑1 (MQ=255) ttttGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTAttt < 1:337505/59‑1 (MQ=255) ttGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACGGtt > 1:1357532/1‑47 (MQ=255) ttGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCa < 1:1226779/66‑1 (MQ=255) ttGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAt > 1:1352992/1‑71 (MQ=255) cacgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAttt < 1:1731619/70‑1 (MQ=255) acaGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTg > 1:1212785/1‑70 (MQ=255) acaGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTg > 1:475595/1‑70 (MQ=255) acaGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTg > 1:1431080/1‑70 (MQ=255) caGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGt > 1:1519329/1‑70 (MQ=255) aGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTaa > 1:1585547/1‑71 (MQ=255) aGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGAt > 1:817831/1‑71 (MQ=255) cccGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCt > 1:1295059/1‑70 (MQ=255) ccGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa < 1:213493/44‑1 (MQ=255) cGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTCTAATGATCACGCTa > 1:1559803/1‑69 (MQ=255) aaCGACCAAAAATAACGGTTTGCATTATTtctctc < 1:1217581/35‑1 (MQ=255) aaCGACCAAAAATAACGGTTTGCATTATTtctctc < 1:1007016/35‑1 (MQ=255) | AATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTA > W3110S.gb/891085‑891217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |