| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 976,718 | G→A | 34.6% | E216E (GAG→GAA) | mukE → | protein involved in chromosome partitioning |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 976,718 | 0 | G | A | 34.6% | 26.5 / 21.5 | 26 | E216E (GAG→GAA) | mukE | protein involved in chromosome partitioning |
| Reads supporting (aligned to +/- strand): ref base G (6/11); new base A (5/4); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
ATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGA > W3110S.gb/976663‑976784 | aTGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGc < 1:1235173/71‑1 (MQ=255) gAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGGATCAGCCAGATAGCGgaga < 1:22063/71‑1 (MQ=255) ttGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGa < 1:1034670/71‑1 (MQ=255) ttGAAAATCATCTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGa > 1:311949/1‑71 (MQ=255) aaTCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGaa < 1:1345507/71‑1 (MQ=255) atcatcTGCAACTCAACGATGAAACCGAAGAAAATCAGCCa < 1:655287/41‑1 (MQ=255) atcTGCAACTCAACGATGAAACCGAAGAAAATCAGCCAGATAGCGgaga < 1:1251575/49‑1 (MQ=255) tcTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCg < 1:322288/44‑1 (MQ=255) gCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGAtt > 1:1627881/1‑60 (MQ=255) cAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGt < 1:575962/68‑1 (MQ=255) aaCTCAACGTTGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAt > 1:1149577/1‑71 (MQ=255) aaCTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAt < 1:350762/71‑1 (MQ=255) aCTCAACGAGGAAACCGCAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGACCGCGGTAAAtt < 1:1307492/71‑1 (MQ=255) cAACGATGAAACCGAAGAAAATCAGCCAGATAGCg > 1:1679509/1‑35 (MQ=255) cAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCg > 1:1013676/1‑71 (MQ=255) cAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCg > 1:1718019/1‑71 (MQ=255) cAACGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCg > 1:497976/1‑71 (MQ=255) aaCGATGAAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc < 1:657875/69‑1 (MQ=255) cGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTc < 1:921989/71‑1 (MQ=255) gATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCa < 1:366728/71‑1 (MQ=255) gATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCa < 1:830969/71‑1 (MQ=255) tGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACt > 1:458258/1‑71 (MQ=255) gAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCg > 1:1748968/1‑64 (MQ=255) gAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACt > 1:710379/1‑70 (MQ=255) aCCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAttt > 1:1383731/1‑59 (MQ=255) aaGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGAGGTAAATTTCGCTCACTGACGCTGa < 1:600839/71‑1 (MQ=255) gagaATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa > 1:1605557/1‑50 (MQ=255) | ATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGA > W3110S.gb/976663‑976784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |