| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,074,318 | G→T | 62.5% | A39E (GCG→GAG) | ycdM ← | predicted monooxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,074,318 | 0 | G | T | 62.5% | ‑3.1 / 18.4 | 16 | A39E (GCG→GAG) | ycdM | predicted monooxygenase |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base T (4/6); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
ATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCT > W3110S.gb/1074254‑1074385 | atGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg > 1:1214246/1‑71 (MQ=255) tGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTggg < 1:971544/64‑1 (MQ=255) tGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTggg < 1:969580/64‑1 (MQ=255) ttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGagccagcc > 1:1692302/1‑71 (MQ=255) ttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCg < 1:512990/70‑1 (MQ=255) caATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGagccagc > 1:21790/1‑63 (MQ=255) aTGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc < 1:1340507/71‑1 (MQ=255) tGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc > 1:458693/1‑70 (MQ=255) gCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCgttgt > 1:1338912/1‑64 (MQ=255) tATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAgg < 1:1066708/70‑1 (MQ=255) aTTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGt < 1:1064716/70‑1 (MQ=255) gTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAAt < 1:867254/71‑1 (MQ=255) cAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACg > 1:1196394/1‑71 (MQ=255) aGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGcc > 1:1238468/1‑70 (MQ=255) gCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCaa < 1:1691562/48‑1 (MQ=255) cTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTtcatcatc < 1:1354738/70‑1 (MQ=255) cggctCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAAc < 1:810852/70‑1 (MQ=255) ggcgCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACct > 1:476963/1‑71 (MQ=255) | ATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCT > W3110S.gb/1074254‑1074385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |