Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,084,947 A→G 64.3% K384E (AAA→GAA)  ycdB → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,084,9470AG64.3% 10.0 / 9.5 14K384E (AAA→GAA) ycdBconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base G (4/5);  total (6/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGC  >  W3110S.gb/1084878‑1084997
                                                                     |                                                  
cAACTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaa                                                   <  1:176316/71‑1 (MQ=255)
              gTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCg                                     <  1:1237919/71‑1 (MQ=255)
              gTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCg                                     <  1:324238/71‑1 (MQ=255)
               ttGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCg                                     <  1:1498301/70‑1 (MQ=255)
                     tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCt                              >  1:748495/1‑71 (MQ=255)
                     tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCt                              >  1:876092/1‑71 (MQ=255)
                       tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTg                             >  1:261405/1‑70 (MQ=255)
                             cTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAAt                      <  1:1705847/71‑1 (MQ=255)
                                ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATa                     <  1:1021693/69‑1 (MQ=255)
                                ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc                                            <  1:1100014/46‑1 (MQ=255)
                                ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc                                            <  1:194118/46‑1 (MQ=255)
                                                    gCTTCCTGACAGTACAAGAAAGGCTCAATGGCGaa                                   >  1:918571/1‑35 (MQ=255)
                                                            aCAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACg                   >  1:150747/1‑43 (MQ=255)
                                                            aCAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcggc  <  1:84520/60‑1 (MQ=255)
                                                                  cAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACg                   >  1:938371/1‑37 (MQ=255)
                                                                     |                                                  
CAACTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGC  >  W3110S.gb/1084878‑1084997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑