| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,088,483 | C→T | 73.9% | Q599Q (CAG→CAA) | ycdR ← | predicted enzyme associated with biofilm formation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,088,483 | 0 | C | T | 73.9% | 34.0 / 12.7 | 23 | Q599Q (CAG→CAA) | ycdR | predicted enzyme associated with biofilm formation |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (10/7); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGC > W3110S.gb/1088418‑1088545 | gCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAgg > 1:1147548/1‑71 (MQ=255) tGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGAt > 1:1341743/1‑71 (MQ=255) gATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGATg > 1:20400/1‑71 (MQ=255) tGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtttt < 1:757231/70‑1 (MQ=255) aTTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTg < 1:32685/54‑1 (MQ=255) tttttCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAgg < 1:1437014/56‑1 (MQ=255) tttCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGAt > 1:1677853/1‑71 (MQ=255) ccAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGATTGGTc < 1:1732802/70‑1 (MQ=255) ccAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGATTGGTc < 1:284713/70‑1 (MQ=255) cAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGAt > 1:934596/1‑50 (MQ=255) cAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGAt > 1:284746/1‑50 (MQ=255) tAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAAttt > 1:829800/1‑47 (MQ=255) tAATTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGATTg > 1:470308/1‑52 (MQ=255) aaTTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg > 1:131255/1‑71 (MQ=255) aaTTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg > 1:1212029/1‑71 (MQ=255) aaTTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg > 1:458353/1‑71 (MQ=255) aaTTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg > 1:677339/1‑71 (MQ=255) aaTTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg > 1:1084682/1‑71 (MQ=255) aTTCTAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTgg > 1:112532/1‑71 (MQ=255) ttCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGt < 1:192368/71‑1 (MQ=255) tAAAATAGATTTGTCTTTAGCTTGAGGGATGTTTTTAAttt < 1:478157/41‑1 (MQ=255) aaaTAGATTTGTCTTTAGCTTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGcc < 1:17449/71‑1 (MQ=255) cTTTAGCTTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGc < 1:783448/70‑1 (MQ=255) | GCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGC > W3110S.gb/1088418‑1088545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |