| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,099,547 | C→T | 61.9% | A143V (GCC→GTC) | ycdX → | predicted zinc‑binding hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,099,547 | 0 | C | T | 61.9% | 9.2 / 20.3 | 21 | A143V (GCC→GTC) | ycdX | predicted zinc‑binding hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base T (5/8); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01 | |||||||||||
ACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTC > W3110S.gb/1099477‑1099613 | aCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGc > 1:1186217/1‑71 (MQ=255) tCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGtt > 1:1056169/1‑71 (MQ=255) cAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTg < 1:1333778/71‑1 (MQ=255) ataataAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGa < 1:1116346/67‑1 (MQ=255) ataataAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAagcag > 1:329719/1‑61 (MQ=255) taataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGcc > 1:1547567/1‑47 (MQ=255) ataaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATc < 1:511375/70‑1 (MQ=255) taaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATc > 1:517399/1‑69 (MQ=255) taaGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGAGAAACATc > 1:306841/1‑69 (MQ=255) ccGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTg > 1:777148/1‑71 (MQ=255) ggAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGa < 1:809185/52‑1 (MQ=255) ggAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCt < 1:722728/68‑1 (MQ=255) ggAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGa < 1:1143516/52‑1 (MQ=255) tGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGaa < 1:1307874/39‑1 (MQ=255) aaaTAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGgcgc > 1:311069/1‑51 (MQ=255) aGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAtttt > 1:690468/1‑71 (MQ=255) gATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAtttt < 1:809993/70‑1 (MQ=255) gATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAtttt < 1:832385/70‑1 (MQ=255) aTGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCTGGTGGCGCTGGAAATCaat < 1:871975/56‑1 (MQ=255) gtgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCt > 1:1683046/1‑44 (MQ=255) gtgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCt > 1:1681036/1‑44 (MQ=255) tgAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTaca > 1:961754/1‑70 (MQ=255) aaaGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTc < 1:156371/71‑1 (MQ=255) | ACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTC > W3110S.gb/1099477‑1099613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |