| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,210,070 | C→T | 53.3% | S121F (TCC→TTC) | ymfS → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,210,070 | 0 | C | T | 53.3% | ‑3.3 / 17.1 | 15 | S121F (TCC→TTC) | ymfS | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base T (5/3); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGC > W3110S.gb/1210000‑1210132 | aaaGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATc > 1:1413339/1‑71 (MQ=255)aaaGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATc > 1:379706/1‑71 (MQ=255) gAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAATgcgc > 1:1431371/1‑70 (MQ=255) cTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGa < 1:692194/71‑1 (MQ=255) cTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTgcgc > 1:1445600/1‑67 (MQ=255) aaCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGATAtt > 1:381331/1‑71 (MQ=255) atatAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGATATTGCAt < 1:754478/71‑1 (MQ=255) taATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGatat > 1:1461387/1‑63 (MQ=255) aTTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCt < 1:935502/71‑1 (MQ=255) tgtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGCCTACg > 1:1584177/1‑71 (MQ=255) gATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGCCTACGCCTCCgg > 1:51937/1‑70 (MQ=255) ttATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAg < 1:1124771/71‑1 (MQ=255) tCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGtt < 1:33190/70‑1 (MQ=255) ggAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGCCTACGc > 1:483519/1‑47 (MQ=255) gAACTGGTTGATACATCCAGTGCGCCAGATATTGaa > 1:1028877/1‑36 (MQ=255) gATACATTCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGgcg > 1:244000/1‑69 (MQ=255) gATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGgcgc > 1:895204/1‑70 (MQ=40) | AAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGC > W3110S.gb/1210000‑1210132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |