| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,224,919 | G→A | 64.3% | intergenic (‑423/‑123) | ymgG ← / → ymgH | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,224,919 | 0 | G | A | 64.3% | 22.2 / 26.1 | 28 | intergenic (‑423/‑123) | ymgG/ymgH | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (6/4); new base A (11/7); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAA > W3110S.gb/1224851‑1224984 | gaTATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTa > 1:1532184/1‑71 (MQ=255)gaTATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTa > 1:598656/1‑71 (MQ=255) catcaGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACa < 1:29583/70‑1 (MQ=255) aaCGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATc < 1:1576192/71‑1 (MQ=255) aaCGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat > 1:588093/1‑70 (MQ=255) aaCGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat > 1:1548666/1‑70 (MQ=255) aTTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:1076439/71‑1 (MQ=255) ttAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGt > 1:1607423/1‑71 (MQ=255) attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACa > 1:1169449/1‑47 (MQ=255) attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAtttt < 1:1448325/71‑1 (MQ=255) ttttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGa < 1:695421/44‑1 (MQ=255) ttttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGa < 1:506148/44‑1 (MQ=255) tttattctGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAttttt > 1:1569423/1‑70 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAagag > 1:849435/1‑61 (MQ=255) attttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATc > 1:1365691/1‑71 (MQ=255) attttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATc > 1:789421/1‑71 (MQ=255) ccTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACtt > 1:493948/1‑69 (MQ=255) ccTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCa < 1:1222084/71‑1 (MQ=255) ccTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAtt < 1:1135274/51‑1 (MQ=255) tCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgc > 1:1764514/1‑71 (MQ=255) aTTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa > 1:875366/1‑57 (MQ=255) ttGTCGAAAGATAAGCATACGACAGTATTATCAGAAAag < 1:580461/39‑1 (MQ=255) ttGTCGAAAGATAAGCATACGACAGTATTATCAGAAAag < 1:303341/39‑1 (MQ=255) gTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCAc > 1:955348/1‑70 (MQ=255) gTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCAc > 1:1516764/1‑70 (MQ=255) aaGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTacac > 1:1734197/1‑55 (MQ=255) aGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCgtgt < 1:689327/71‑1 (MQ=255) aaGCATACGACAGTATTATCAGAAAAGAGTGATTTTATATCCAACTACACTTCAGCGCACTGCGTGTaaa > 1:180456/1‑70 (MQ=255) | GATATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAA > W3110S.gb/1224851‑1224984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |