| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,255,918 | C→T | 41.7% | G538S (GGC→AGC) | ycgV ← | predicted adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,255,918 | 0 | C | T | 41.7% | 10.7 / 25.6 | 24 | G538S (GGC→AGC) | ycgV | predicted adhesin |
| Reads supporting (aligned to +/- strand): ref base C (7/7); new base T (3/7); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
AGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAA > W3110S.gb/1255859‑1255984 | aGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAg < 1:556848/71‑1 (MQ=255) ctCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCa > 1:1713845/1‑70 (MQ=255) ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataa > 1:668830/1‑70 (MQ=255) tCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTg > 1:560504/1‑71 (MQ=255) aaTAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGt < 1:795790/70‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt < 1:141593/45‑1 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc > 1:1643507/1‑71 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc < 1:324269/71‑1 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATAAGCACGCATAATAAAGGTACTGTTAc > 1:1156994/1‑71 (MQ=255) cTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg < 1:1635134/71‑1 (MQ=255) cTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataa < 1:395724/48‑1 (MQ=255) attaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTctct < 1:840020/70‑1 (MQ=255) taACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTg > 1:1576491/1‑50 (MQ=255) gCCATTACCCTCGCCAACAACATCAGCACGCataat > 1:358547/1‑36 (MQ=255) gCCATTACCCTCGCCAACAACATCAGCACGCataat > 1:1741845/1‑36 (MQ=255) gCCATTACCCTCGCCAACAACATCAGCACGCataat > 1:1617955/1‑36 (MQ=255) aTTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg < 1:1223456/71‑1 (MQ=255) ttACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa < 1:456287/39‑1 (MQ=255) aCCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg > 1:1387337/1‑70 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg < 1:366408/61‑1 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg < 1:1209761/61‑1 (MQ=255) tCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg < 1:1749047/48‑1 (MQ=255) tCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg < 1:416485/48‑1 (MQ=255) gCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaaa < 1:48876/69‑1 (MQ=255) | AGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAA > W3110S.gb/1255859‑1255984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |