| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,274,854 | G→A | 58.3% | T108M (ACG→ATG) | ychN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,274,854 | 0 | G | A | 58.3% | 6.8 / 26.4 | 24 | T108M (ACG→ATG) | ychN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/7); new base A (6/8); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
CCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTG > W3110S.gb/1274788‑1274911 | cccGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTc < 1:1029312/69‑1 (MQ=255)cccGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTc < 1:63376/69‑1 (MQ=255)cccGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTc < 1:1255941/69‑1 (MQ=255) aGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACaa < 1:947235/70‑1 (MQ=255) aGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACaa < 1:135970/70‑1 (MQ=255) cccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAg > 1:637200/1‑55 (MQ=255) cccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCg < 1:1485711/71‑1 (MQ=255) cTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACAGAt > 1:1113009/1‑71 (MQ=255) tCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATc < 1:1185799/41‑1 (MQ=255) cAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGAttt > 1:936238/1‑71 (MQ=255) aaaCATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGAtt < 1:1089084/69‑1 (MQ=255) aCATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCa > 1:1125103/1‑39 (MQ=255) aCATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCa > 1:594824/1‑39 (MQ=255) aTTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAc > 1:1308797/1‑70 (MQ=255) ttAAAATGTGAGCACTTTATCGGCTGACAGCATcc < 1:300699/35‑1 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCa > 1:472642/1‑42 (MQ=255) aGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatca < 1:897662/70‑1 (MQ=255) tttATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc < 1:1229459/45‑1 (MQ=255) tttATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAgg > 1:137982/1‑69 (MQ=255) tATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGAt > 1:1284842/1‑46 (MQ=255) tATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAg < 1:908573/70‑1 (MQ=255) tCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAgtg < 1:805195/70‑1 (MQ=255) tCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAgtg < 1:791648/70‑1 (MQ=255) aCAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAgg < 1:1028513/58‑1 (MQ=255) | CCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTG > W3110S.gb/1274788‑1274911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |