Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,413,523 G→A 41.7% S47F (TCC→TTC)  ydaO ← predicted C32 tRNA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,413,5230GA41.7% 2.0 / 10.0 12S47F (TCC→TTC) ydaOpredicted C32 tRNA thiolase
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base A (3/2);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTA  >  W3110S.gb/1413472‑1413591
                                                   |                                                                    
tgctgcAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatgatg                                                     <  1:536243/69‑1 (MQ=255)
      aaaTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGcc                                             <  1:215516/71‑1 (MQ=255)
            cgcAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCcttctt                                        <  1:1271520/70‑1 (MQ=255)
            cgcAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCcttctt                                        <  1:948322/70‑1 (MQ=255)
               aGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCat                                                         <  1:1077461/50‑1 (MQ=255)
                 aaTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatga                                                       >  1:1468040/1‑50 (MQ=255)
                 aaTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatga                                                       >  1:96613/1‑50 (MQ=255)
                                      gTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCtt             >  1:1575710/1‑71 (MQ=255)
                                       tCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCtt             <  1:95331/70‑1 (MQ=255)
                                         tttACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCa                       >  1:837365/1‑58 (MQ=255)
                                              ccccGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACg     >  1:1117701/1‑71 (MQ=255)
                                                 cGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTa  >  1:1713326/1‑71 (MQ=255)
                                                   |                                                                    
TGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTA  >  W3110S.gb/1413472‑1413591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑