| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,484,463 | T→C | 44.4% | I38V (ATC→GTC) | azoR ← | NADH‑azoreductase, FMN‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,484,463 | 0 | T | C | 44.4% | 0.1 / 20.7 | 18 | I38V (ATC→GTC) | azoR | NADH‑azoreductase, FMN‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (4/6); new base C (3/5); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGAG > W3110S.gb/1484393‑1484528 | ggACGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGAt < 1:1734161/71‑1 (MQ=255) aGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCtt > 1:1214079/1‑71 (MQ=255) cATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCgcgc < 1:1710308/71‑1 (MQ=255) aTCCAGCACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCgcgc > 1:725893/1‑70 (MQ=255) tACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGtt < 1:1321565/71‑1 (MQ=255) aCCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGa > 1:1695726/1‑50 (MQ=255) aCCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGtt > 1:536668/1‑70 (MQ=255) aCCGGAATAGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGa > 1:1120908/1‑50 (MQ=255) tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaaca < 1:1353936/68‑1 (MQ=255) tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACaaaa < 1:423124/71‑1 (MQ=255) tttGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATc > 1:448418/1‑71 (MQ=255) gCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCgg < 1:1259768/70‑1 (MQ=255) cAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaata < 1:677105/60‑1 (MQ=255) gcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTa > 1:612704/1‑70 (MQ=255) aCGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTg < 1:220705/71‑1 (MQ=255) cGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTga < 1:752851/71‑1 (MQ=255) ggTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa < 1:360398/44‑1 (MQ=255) ggTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTgag < 1:1601638/71‑1 (MQ=255) ggTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTgag < 1:1283014/71‑1 (MQ=255) | GGACGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGAG > W3110S.gb/1484393‑1484528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |