Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,584,624 A→G 100% intergenic (‑386/+16) ydeN ← / ← ydeO conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,584,6240AG76.5% 29.0 / 5.6 17intergenic (‑386/+16)ydeN/ydeOconserved hypothetical protein/predicted DNA‑binding transcriptional acfivator
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (10/3);  total (11/6)
Fisher's exact test for biased strand distribution p-value = 9.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGT  >  W3110S.gb/1584572‑1584686
                                                    |                                                              
gggCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCa                                              <  1:89619/71‑1 (MQ=255)
      ggAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCaaa                                            >  1:1498401/1‑67 (MQ=255)
       gAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGc                                        >  1:1638454/1‑70 (MQ=255)
        aaTGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTa                                      <  1:507854/71‑1 (MQ=255)
         aTGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTa                                      >  1:1304365/1‑70 (MQ=255)
            gCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGc                                  <  1:567950/71‑1 (MQ=255)
                  ccATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCAt                            >  1:840084/1‑71 (MQ=255)
                  ccATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCAt                            >  1:654646/1‑71 (MQ=255)
                      ttAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                        >  1:492064/1‑71 (MQ=255)
                      ttAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                        >  1:207194/1‑71 (MQ=255)
                       tAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCaa                                             >  1:1666462/1‑49 (MQ=255)
                        aaTTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTaa                                     >  1:1251791/1‑56 (MQ=255)
                         aTTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAaat                                                   >  1:653183/1‑41 (MQ=255)
                         aTTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCa                                 >  1:1243572/1‑59 (MQ=255)
                         aTTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGtt                      <  1:632804/70‑1 (MQ=255)
                                             gTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCgtgt  <  1:223642/70‑1 (MQ=255)
                                             gTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGACCGTATTCATACCCgtgt  <  1:614197/70‑1 (MQ=255)
                                                    |                                                              
GGGCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGT  >  W3110S.gb/1584572‑1584686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑