| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,590,693 | A→G | 16.0% | L341L (CTT→CTC) | ydeT ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,590,693 | 0 | A | G | 16.0% | 56.4 / 5.5 | 25 | L341L (CTT→CTC) | ydeT | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (11/10); new base G (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
GTACTCGACAATACAGTTTGAGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTATTCTCTCCGTGTGTGACAA > W3110S.gb/1590627‑1590763 | gTACTCGACAATACAGTTTGAGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGGAGTc > 1:477091/1‑71 (MQ=255) aTACAGTTTGAGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATa < 1:1072644/71‑1 (MQ=255) aGTTTGAGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAAcc > 1:136766/1‑71 (MQ=255) gTTTGAGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCt < 1:1549907/71‑1 (MQ=255) gAGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGAc > 1:1499381/1‑70 (MQ=255) tttATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAAc > 1:1436505/1‑60 (MQ=255) ttATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTc > 1:949778/1‑71 (MQ=255) aTCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTc > 1:1332898/1‑69 (MQ=255) tttGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGa > 1:203598/1‑71 (MQ=255) tGCCCCATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGAc > 1:1210881/1‑70 (MQ=255) tGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCcgcg < 1:199992/68‑1 (MQ=255) tGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCcgc < 1:242396/67‑1 (MQ=255) ccATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAAtgc < 1:174344/71‑1 (MQ=255) gAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGAc < 1:107305/49‑1 (MQ=255) ccTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCAttt > 1:953248/1‑71 (MQ=255) ttGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACaa < 1:827175/53‑1 (MQ=255) ttGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAAtg < 1:1248142/55‑1 (MQ=255) tGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGa > 1:639459/1‑36 (MQ=255) gCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAAtgct > 1:1215027/1‑55 (MQ=255) cTGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACaa < 1:138584/48‑1 (MQ=255) cTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTATTCTCTCCg < 1:1113316/71‑1 (MQ=255) cTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTATTCTCTCCg < 1:398063/71‑1 (MQ=255) cTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTATTCTCTCCg > 1:124526/1‑71 (MQ=255) tGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTTCCATTTTTATTCTCTCCgt > 1:1230949/1‑71 (MQ=255) gAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTATTCTCTCCGTGTGTGAc < 1:867034/70‑1 (MQ=255) aaGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTATTCTCTCCGTGTGTGACaa > 1:1025636/1‑71 (MQ=255) | GTACTCGACAATACAGTTTGAGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTATTCTCTCCGTGTGTGACAA > W3110S.gb/1590627‑1590763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |