| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,693,796 | C→T | 52.9% | G257D (GGC→GAC) | uidC ← | predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,693,796 | 0 | C | T | 52.9% | ‑5.2 / 21.1 | 17 | G257D (GGC→GAC) | uidC | predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base C (4/4); new base T (5/4); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
CGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTAT > W3110S.gb/1693741‑1693863 | cGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGTCGAGCAACTCCCCtt > 1:1690243/1‑71 (MQ=255)cGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGTCGAGCAACTCCCCt < 1:1650468/70‑1 (MQ=255)cGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCtt < 1:1177986/71‑1 (MQ=255) ttCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTc > 1:291160/1‑70 (MQ=255) cATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGTCGAGCAACTCCCCTTTCAAc > 1:948323/1‑71 (MQ=255) tggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGTCGAGc < 1:1654639/44‑1 (MQ=255) gTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGcc < 1:1022695/38‑1 (MQ=255) aCGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCg > 1:653688/1‑71 (MQ=255) cTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTAt > 1:1397516/1‑71 (MQ=255) ttAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCgg < 1:712327/67‑1 (MQ=255) aaaCCTTCGAGCTGCGCGTAAAAGTCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGc < 1:1106911/70‑1 (MQ=255) aaaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAAc < 1:778301/45‑1 (MQ=255) ttCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAg < 1:1369834/57‑1 (MQ=255) tCGAGCTGCGCGTAAAAGTCGCGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTtctc > 1:597914/1‑71 (MQ=255) tCGAGCTGCGCGTAAAAGTCGAGCAACTCCCCTTTCAACGTATAAtt > 1:1403806/1‑47 (MQ=255) tAAAAGTCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACtt < 1:302469/69‑1 (MQ=255) tAAAAGTCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTg > 1:289389/1‑71 (MQ=255) aaGCCGAGCAACTCGCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTAt > 1:556714/1‑71 (MQ=255) | CGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTAT > W3110S.gb/1693741‑1693863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |