| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,721,565 | T→C | 25.0% | intergenic (‑249/‑25) | ydhH ← / → slyB | conserved hypothetical protein/outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,721,565 | 0 | T | C | 25.0% | 46.0 / 14.6 | 28 | intergenic (‑249/‑25) | ydhH/slyB | conserved hypothetical protein/outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (13/8); new base C (4/3); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGAACATGTAACCATGGATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGGTCTGTC > W3110S.gb/1721498‑1721630 | gTAGAACATGTAACCATGGATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTc < 1:1634499/70‑1 (MQ=255) cATGTAACCATGGATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTg > 1:1347073/1‑70 (MQ=255) aTGTAACCATGGATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGaa > 1:1062585/1‑71 (MQ=255) tAACCATGGATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAg < 1:167263/71‑1 (MQ=255) gATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATg > 1:428156/1‑55 (MQ=255) gATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGAtttt > 1:1725341/1‑71 (MQ=255) tCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCa < 1:213122/50‑1 (MQ=255) tGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCaa < 1:895178/65‑1 (MQ=255) tGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGAtt > 1:78837/1‑70 (MQ=255) ccATATACTTTGACCATGAGAGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTaaa < 1:634075/71‑1 (MQ=255) atatACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACa > 1:913490/1‑48 (MQ=255) atatACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAAcg > 1:1412211/1‑71 (MQ=255) tataCTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATgg > 1:1516739/1‑41 (MQ=255) tataCTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGAtt < 1:1492573/65‑1 (MQ=255) ataCTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAAcg < 1:878076/69‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAAcgcg > 1:1511810/1‑70 (MQ=255) aCCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGgtt < 1:299113/71‑1 (MQ=255) tGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGttt > 1:570867/1‑71 (MQ=255) tGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGttt > 1:1218772/1‑71 (MQ=255) gggATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAATGCGTATTGGTTGTTTCaa > 1:1297252/1‑71 (MQ=255) gggATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCaa > 1:221143/1‑71 (MQ=255) tGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGt < 1:340297/71‑1 (MQ=255) cTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGgttgt < 1:267463/60‑1 (MQ=255) gCGTGGCGTCTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCa < 1:432470/61‑1 (MQ=255) ggCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGgtctgtc > 1:242723/1‑71 (MQ=255) gCGTCTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGgtct > 1:1618176/1‑67 (MQ=255) cGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGgt > 1:1671012/1‑64 (MQ=255) cGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGgt > 1:942106/1‑64 (MQ=255) | GTAGAACATGTAACCATGGATTCACATATGCCATATACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGGTCTGTC > W3110S.gb/1721498‑1721630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |