Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,857,513 C→T 29.0% G58E (GGA→GAA)  ydjG ← predicted oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,857,5130CT29.0% 45.4 / 20.0 31G58E (GGA→GAA) ydjGpredicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (11/11);  new base T (4/5);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGGGCAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATA  >  W3110S.gb/1857455‑1857572
                                                          |                                                           
aCGGGGCAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTat                                                 <  1:54435/71‑1 (MQ=255)
      cAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTatcaatca                                           <  1:1549061/71‑1 (MQ=255)
        gTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa                                         <  1:112019/71‑1 (MQ=255)
        gTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa                                         <  1:1229704/71‑1 (MQ=255)
         tttttttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGa                                         <  1:986422/70‑1 (MQ=255)
           tttttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTg                                                          >  1:340506/1‑51 (MQ=255)
           tttttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGAtt                                       >  1:517425/1‑70 (MQ=255)
           tttttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGAtt                                       >  1:1430788/1‑70 (MQ=255)
           tttttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGAt                                        >  1:1374356/1‑69 (MQ=255)
              ttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAAt                                    <  1:415692/70‑1 (MQ=255)
                 aCGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGgcgc                                                      <  1:1709757/49‑1 (MQ=255)
                 aCGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGATTa                                      <  1:395998/65‑1 (MQ=255)
                        aCCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGAt                         >  1:100874/1‑71 (MQ=255)
                         ccGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTaa                                     <  1:1008805/58‑1 (MQ=255)
                            acgaTAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGc                     >  1:660747/1‑71 (MQ=255)
                            acgaTAACTTCAATATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGAtt                                       <  1:1104412/53‑1 (MQ=255)
                                tAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCa                 <  1:1236946/71‑1 (MQ=255)
                                  aCTTCACTATTGCCAAAGTTATATCCTGGCGCAGTat                                                 >  1:460381/1‑37 (MQ=255)
                                    ttCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTa                                      <  1:560459/46‑1 (MQ=255)
                                      cACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAg               >  1:65806/1‑67 (MQ=255)
                                      cACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGa              >  1:921644/1‑68 (MQ=255)
                                      cACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAAt            <  1:761501/70‑1 (MQ=255)
                                        cTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAAc                            <  1:640995/52‑1 (MQ=255)
                                        cTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCg          >  1:607394/1‑70 (MQ=255)
                                         tATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACg                           <  1:954913/52‑1 (MQ=255)
                                         tATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACg                           <  1:307320/52‑1 (MQ=255)
                                            tGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATc      >  1:721342/1‑70 (MQ=255)
                                              ccAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGa                          >  1:1284293/1‑48 (MQ=255)
                                                aaaGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATa  >  1:869/1‑70 (MQ=255)
                                                   gTTATATTCTGGCGCAGTATCAATCAGATTAATGCcaca                              >  1:802018/1‑39 (MQ=255)
                                                   gTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTa        >  1:894939/1‑61 (MQ=255)
                                                          |                                                           
ACGGGGCAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATA  >  W3110S.gb/1857455‑1857572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: