| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,872,074 | T→C | 16.0% | intergenic (+122/‑25) | yeaH → / → yeaI | conserved hypothetical protein/predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,872,074 | 0 | T | C | 16.0% | 56.3 / 5.7 | 25 | intergenic (+122/‑25) | yeaH/yeaI | conserved hypothetical protein/predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (12/9); new base C (2/2); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTT > W3110S.gb/1872009‑1872136 | cATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTc > 1:1486417/1‑68 (MQ=255) ttaCATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAAc < 1:891682/70‑1 (MQ=255) tacatTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt > 1:478952/1‑71 (MQ=255) catTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc > 1:27549/1‑71 (MQ=255) gCTTACAATCGCTTTAAATATGACAGGATAACCTTTACATAATTTAGTTCCAGaaaa > 1:998209/1‑57 (MQ=255) aCAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaaa < 1:189439/69‑1 (MQ=255) cAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGa > 1:1430679/1‑71 (MQ=255) aTCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt < 1:711043/71‑1 (MQ=255) tCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAtt < 1:371462/57‑1 (MQ=255) tttAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa < 1:678395/68‑1 (MQ=255) tAAATATGACAGCATAACCTTTACATAATTTAGTTc < 1:1608988/36‑1 (MQ=255) aaaTATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCaa > 1:1658637/1‑70 (MQ=255) aaTATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATc > 1:582417/1‑46 (MQ=255) aaTATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATc > 1:91733/1‑46 (MQ=255) atatGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc < 1:435271/66‑1 (MQ=255) atGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAAt < 1:1626930/42‑1 (MQ=255) tGACAGCATAACCTTTACATAATTTAGCTCCAGAAAACAAt < 1:1608264/41‑1 (MQ=255) aCAGCATAACCTTTACATAATTTAGCTCCAGAAAACAATCa > 1:1750610/1‑41 (MQ=255) tAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCaa < 1:959006/57‑1 (MQ=255) tAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATg > 1:608581/1‑71 (MQ=255) tAACCTTTACATAATTTAGCTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATg < 1:285116/71‑1 (MQ=255) aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCa > 1:374978/1‑67 (MQ=255) aCATAATTTAGCTCCAGAAAACAATCATTCGGAAAAATGa > 1:573715/1‑40 (MQ=255) tAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCtt > 1:1694647/1‑71 (MQ=255) tAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCtt > 1:1186469/1‑71 (MQ=255) | CATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTT > W3110S.gb/1872009‑1872136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |