| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,176,396 | C→T | 72.4% | M6I (ATG→ATA) | gatC ← | galactitol‑specific enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,176,396 | 0 | C | T | 72.4% | 42.8 / 17.5 | 29 | M6I (ATG→ATA) | gatC | galactitol‑specific enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (2/6); new base T (9/12); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGA > W3110S.gb/2176330‑2176460 | tatTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCAt < 1:589589/69‑1 (MQ=255) aaaaTAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGaaaa > 1:891240/1‑71 (MQ=255) aataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACa < 1:370495/71‑1 (MQ=255) ataatGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACAt < 1:533030/71‑1 (MQ=255) gaCAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGa > 1:1228481/1‑50 (MQ=255) cAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCAcccc > 1:1569815/1‑71 (MQ=255) tCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATa > 1:47659/1‑59 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTaa < 1:575538/71‑1 (MQ=255) ggCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACCCATGACTTCTGAAAACATAGGTCACCCCTGTa < 1:1399092/70‑1 (MQ=255) gcaTCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGaa < 1:665718/47‑1 (MQ=255) tCACCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAg < 1:850875/65‑1 (MQ=255) tCACCGTAGGGCCGAGGTCGAGAATATAACGCATg < 1:1675322/35‑1 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGa < 1:471199/41‑1 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACAt < 1:384523/47‑1 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACAt < 1:319920/47‑1 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGAt > 1:782859/1‑65 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGAt > 1:1377094/1‑65 (MQ=255) aCCGTAGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAg < 1:831481/71‑1 (MQ=255) aGGGCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACa > 1:494460/1‑41 (MQ=255) aGGCCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACa > 1:1100813/1‑41 (MQ=255) ggCCGAGGTCGAGAATATAACGTATGACTTCTGAAAACATAg < 1:479171/42‑1 (MQ=255) aGGTCGAGAATATAACGTATGACTTCTGAAAACATAGGTCAcc < 1:61335/43‑1 (MQ=255) ataACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTcga > 1:1235593/1‑71 (MQ=255) taACGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGa < 1:775251/40‑1 (MQ=255) aaCGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTg < 1:76766/58‑1 (MQ=255) aaCGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTg < 1:1098209/58‑1 (MQ=255) aCGTATGACTTCTGAAAACATAGGTCACCCCTGTAAGATa > 1:649573/1‑40 (MQ=255) gCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATa < 1:1720994/38‑1 (MQ=255) gCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAatttt > 1:774783/1‑49 (MQ=255) | TATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGA > W3110S.gb/2176330‑2176460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |