| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,218,928 | T→C | 29.6% | I243I (ATT→ATC) | mlrA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,218,928 | 0 | T | C | 29.6% | 33.3 / 18.9 | 27 | I243I (ATT→ATC) | mlrA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (6/13); new base C (2/6); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
AACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAT > W3110S.gb/2218859‑2218989 | aaCCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCAttt < 1:1489339/71‑1 (MQ=255) aaCAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGt > 1:1006297/1‑70 (MQ=255) aaCAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGt > 1:467979/1‑70 (MQ=255) cagcagCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTaa > 1:1095658/1‑71 (MQ=255) aaCTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTa < 1:280472/52‑1 (MQ=255) cTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACAAATgcgc > 1:138629/1‑71 (MQ=255) aCCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACAAATGCGCtt < 1:1677388/70‑1 (MQ=255) ccAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACa < 1:256522/60‑1 (MQ=255) gTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTg < 1:1060116/71‑1 (MQ=255) ttGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAAt < 1:317472/44‑1 (MQ=255) ttGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAAt < 1:608630/44‑1 (MQ=255) ggCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTa < 1:1754794/70‑1 (MQ=255) ggCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTa > 1:32675/1‑70 (MQ=255) ctaGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGAtt < 1:905312/42‑1 (MQ=255) cAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACaa > 1:1520997/1‑71 (MQ=255) aaGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTa < 1:274700/48‑1 (MQ=255) aaGAACAAGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTa < 1:704313/48‑1 (MQ=255) aaGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACaa < 1:1466404/46‑1 (MQ=255) aaGGCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACAAATGCGCTTTACTGTAc < 1:1732811/62‑1 (MQ=255) gCCATGATATTTTCCCACTCGGCATCTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGtt < 1:647228/71‑1 (MQ=255) tGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCtt < 1:1771332/47‑1 (MQ=255) atatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACAt < 1:35399/70‑1 (MQ=255) atatTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATa < 1:948171/71‑1 (MQ=255) atTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACAt > 1:129296/1‑68 (MQ=255) ttttCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCtt < 1:634838/42‑1 (MQ=255) ccACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTg < 1:586485/70‑1 (MQ=255) aCTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAt < 1:1230152/71‑1 (MQ=255) cTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAt > 1:1488040/1‑70 (MQ=255) | AACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCAT > W3110S.gb/2218859‑2218989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |