| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,238,432 | T→C | 100% | L356L (TTA→CTA) | yeiT → | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,238,432 | 0 | T | C | 88.2% | 45.0 / ‑0.7 | 17 | L356L (TTA→CTA) | yeiT | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (9/6); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTGC > W3110S.gb/2238366‑2238496 | aCGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTag < 1:1749317/70‑1 (MQ=255) gcggcAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCa < 1:1326306/71‑1 (MQ=255) cggcAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAg < 1:1397427/71‑1 (MQ=255) aaaaTTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAacac > 1:1770467/1‑71 (MQ=255) aaaaTTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAacac > 1:412331/1‑71 (MQ=255) attCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCa < 1:1421138/70‑1 (MQ=255) ccGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca > 1:191010/1‑70 (MQ=255) cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc > 1:1731625/1‑69 (MQ=255) aGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTa > 1:535102/1‑67 (MQ=255) cAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcg < 1:1093634/71‑1 (MQ=255) gCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:466973/1‑47 (MQ=255) cTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAAtt < 1:293611/47‑1 (MQ=255) cTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgaccc > 1:999885/1‑56 (MQ=255) ttGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCttt > 1:854913/1‑71 (MQ=255) tGCGGAGTTAGAGCCGCAGCGTAACACCATCaaaa > 1:1422005/1‑35 (MQ=255) tGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTtg > 1:1336560/1‑71 (MQ=255) cGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTtgc < 1:691177/70‑1 (MQ=255) | ACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTGC > W3110S.gb/2238366‑2238496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |