| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,266,989 | T→C | 52.2% | intergenic (‑160/‑208) | fruB ← / → setB | fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,266,989 | 0 | T | C | 52.2% | ‑0.4 / 29.4 | 23 | intergenic (‑160/‑208) | fruB/setB | fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base T (8/3); new base C (10/2); total (18/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAATT > W3110S.gb/2266920‑2267056 | aaaCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGtt > 1:961941/1‑71 (MQ=255) cGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGa > 1:1672081/1‑71 (MQ=255) aaGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGtgct > 1:1595834/1‑71 (MQ=255) gAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAt > 1:981736/1‑64 (MQ=255) aaGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGca > 1:974700/1‑70 (MQ=255) taataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAAttt > 1:1149053/1‑71 (MQ=255) taCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAtttttt > 1:414894/1‑71 (MQ=255) cTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtg > 1:755128/1‑50 (MQ=255) tCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTc > 1:229423/1‑71 (MQ=255) cAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTc < 1:1217056/70‑1 (MQ=255) aaGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCt < 1:809074/71‑1 (MQ=255) ggAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTa < 1:301873/63‑1 (MQ=255) gAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGAt < 1:337166/71‑1 (MQ=255) gCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATggg > 1:1539400/1‑70 (MQ=255) gTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGaaaa > 1:1445215/1‑71 (MQ=255) ttATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCAtttt < 1:763207/44‑1 (MQ=255) tATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTa > 1:60065/1‑71 (MQ=255) aTGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTAt > 1:1628225/1‑71 (MQ=255) aTGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTAt > 1:1161853/1‑71 (MQ=255) aTGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAAt > 1:111728/1‑49 (MQ=255) aTGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAAt > 1:162595/1‑49 (MQ=255) tGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGAt > 1:1676105/1‑55 (MQ=255) aaaGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAat > 1:350034/1‑71 (MQ=255) aaGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAatt > 1:1405069/1‑71 (MQ=255) | AAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAATT > W3110S.gb/2266920‑2267056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |