| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,358,400 | G→A | 57.1% | A362T (GCT→ACT) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,358,400 | 0 | G | A | 57.1% | 3.2 / 24.2 | 21 | A362T (GCT→ACT) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (3/6); new base A (4/8); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
AACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGC > W3110S.gb/2358335‑2358470 | aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:687601/69‑1 (MQ=255)aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGa < 1:596324/70‑1 (MQ=255) cgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATggg < 1:578872/71‑1 (MQ=255) gTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc < 1:596093/70‑1 (MQ=255) gACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGaa > 1:928114/1‑57 (MQ=255) gACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGaa > 1:632569/1‑57 (MQ=255) ttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATg < 1:1656786/70‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGcc < 1:1109487/70‑1 (MQ=255) catcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAAc > 1:901756/1‑71 (MQ=255) caccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTa < 1:1268568/58‑1 (MQ=255) accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg < 1:1092618/52‑1 (MQ=255) ggCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAacac > 1:113488/1‑71 (MQ=255) cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcgc < 1:1405304/71‑1 (MQ=255) cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcgc < 1:889582/71‑1 (MQ=255) aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCaaa > 1:1618275/1‑57 (MQ=255) aCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGcc < 1:691330/71‑1 (MQ=255) cTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGa < 1:694411/71‑1 (MQ=255) cGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTg > 1:873192/1‑45 (MQ=255) gATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:843778/35‑1 (MQ=255) aTGACTGAATGGGCTACCGACGCGGTATGCCGCaa > 1:1054895/1‑35 (MQ=255) aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCt < 1:247552/71‑1 (MQ=255) gCTGAATGGGCTACCTACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGc < 1:715609/71‑1 (MQ=255) | AACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGC > W3110S.gb/2358335‑2358470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |