| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,389,320 | T→C | 16.7% | N358S (AAC→AGC) | yfbK ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,389,320 | 0 | T | C | 16.7% | 66.3 / 8.5 | 30 | N358S (AAC→AGC) | yfbK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (12/13); new base C (3/2); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
AGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTG > W3110S.gb/2389253‑2389382 | agagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGt < 1:1302804/71‑1 (MQ=255) gTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGc > 1:1625547/1‑70 (MQ=255) tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTAtt < 1:1137817/71‑1 (MQ=255) tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTAtt < 1:1438493/71‑1 (MQ=255) gTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCa > 1:1735127/1‑71 (MQ=255) gTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACg < 1:533183/71‑1 (MQ=255) gTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACg < 1:1337508/71‑1 (MQ=255) gTTACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACa < 1:584408/71‑1 (MQ=255) aCCAACATCGGCAATTCGCACCATCATTGCCTCGtt > 1:1612551/1‑36 (MQ=255) aCCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACagag > 1:1342299/1‑71 (MQ=255) ccAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACagag < 1:1696029/70‑1 (MQ=255) ccAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACagag < 1:1694052/70‑1 (MQ=255) aCATCGGCAATTCGCAGCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTaa > 1:454061/1‑70 (MQ=255) aTCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTaa > 1:1146318/1‑68 (MQ=255) aTCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTaa > 1:329122/1‑68 (MQ=255) aTCGGCAATTCGCACCATCAATGCCTCGTTGTAATTGCTATTCCCAACGCCAAACGTCGACAGAGTaa > 1:551237/1‑68 (MQ=255) tCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAacaca < 1:1718047/71‑2 (MQ=255) cGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTaa > 1:1054080/1‑66 (MQ=255) aaTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCCCGCCAAACGTCGACAGAGTAaccc > 1:862633/1‑63 (MQ=255) aaTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACAcc > 1:1132814/1‑66 (MQ=255) cGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCg > 1:1440928/1‑71 (MQ=255) cGCACCATCATTGCCTCGTTGTAATTCCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTccc < 1:262148/70‑1 (MQ=255) atcatTGCCTCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGttt < 1:982641/71‑1 (MQ=255) atTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTaa > 1:1014602/1‑49 (MQ=255) atTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTaa > 1:1771379/1‑49 (MQ=255) atTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtttttt > 1:1442294/1‑71 (MQ=255) atTGCCTCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTaa > 1:1403179/1‑49 (MQ=255) ttGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTc < 1:554608/58‑1 (MQ=255) ttGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTc < 1:98286/58‑1 (MQ=255) ttGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTg < 1:1003590/71‑1 (MQ=255) gCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAac < 1:844330/47‑1 (MQ=255) ttGTAATTGCTATTCCACACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGttt < 1:449973/59‑1 (MQ=255) | AGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTG > W3110S.gb/2389253‑2389382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |