| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,433,800 | G→A | 16.7% | T91T (ACC→ACT) | ubiX ← | 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,433,800 | 0 | G | A | 16.7% | 72.6 / 7.7 | 30 | T91T (ACC→ACT) | ubiX | 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (16/9); new base A (3/2); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
TCAGCACCACATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCA > W3110S.gb/2433736‑2433869 | tCAGCACCACATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGAGtttt > 1:1552725/1‑70 (MQ=255)tCAGCACCACATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGAGtttt > 1:1171146/1‑70 (MQ=255) cAGCACCACATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTttga > 1:1310978/1‑71 (MQ=255) ccacATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGaa < 1:209909/71‑1 (MQ=255) cacaTCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGaa > 1:990123/1‑70 (MQ=255) acaTCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACa < 1:798068/71‑1 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGttt > 1:730229/1‑56 (MQ=255) ccGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTaaa < 1:102048/71‑1 (MQ=255) ccGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTaaa < 1:104146/71‑1 (MQ=255) ccGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTaa > 1:297142/1‑70 (MQ=255) ggTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCa < 1:680382/71‑1 (MQ=255) aGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATccc > 1:1206794/1‑71 (MQ=255) aGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGAGTTTTGATTGAACAGGGTAAAATCACCATccc > 1:1130411/1‑71 (MQ=255) tAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGTGTAAAATc < 1:910864/61‑1 (MQ=255) aaGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAg > 1:844078/1‑71 (MQ=255) aGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTttga > 1:238912/1‑42 (MQ=255) aGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTatga > 1:1461711/1‑42 (MQ=255) tCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAAt < 1:1476133/53‑1 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTttgat > 1:924218/1‑36 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTttgat > 1:1166075/1‑36 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTttgat > 1:598510/1‑36 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGa < 1:1222263/71‑1 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGAGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGa < 1:57706/71‑1 (MQ=255) taGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAg > 1:1836/1‑39 (MQ=255) taGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCAc > 1:231563/1‑50 (MQ=255) taGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg > 1:142119/1‑70 (MQ=255) tGGACAATGCCGGAAAGAGTTTTGATTGAACAGGGTaaaa < 1:1351146/40‑1 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCgg > 1:565932/1‑68 (MQ=255) aCAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:788110/71‑1 (MQ=255) ccGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATg > 1:505465/1‑69 (MQ=255) gggTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCa < 1:1464605/71‑1 (MQ=255) | TCAGCACCACATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCA > W3110S.gb/2433736‑2433869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |