| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,462,102 | A→G | 42.9% | F53L (TTC→CTC) | sixA ← | phosphohistidine phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,462,102 | 0 | A | G | 42.9% | 2.7 / 24.0 | 21 | F53L (TTC→CTC) | sixA | phosphohistidine phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (4/8); new base G (4/5); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
CACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTC > W3110S.gb/2462042‑2462171 | cACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCAcc < 1:967031/71‑1 (MQ=255)cACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCAcc < 1:1693096/71‑1 (MQ=255) cTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAg < 1:387170/71‑1 (MQ=255) cTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAg < 1:658870/71‑1 (MQ=255) gAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGaa > 1:1313085/1‑56 (MQ=255) gAAGGCAGGTTCAGACAATCCACTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGaa > 1:412779/1‑56 (MQ=255) gCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGt < 1:362303/71‑1 (MQ=255) ggTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCg < 1:946101/71‑1 (MQ=255) cAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGAttt > 1:723144/1‑71 (MQ=255) aGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGAttt < 1:1664803/70‑1 (MQ=255) aGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGAttt < 1:118701/70‑1 (MQ=255) ttcttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCtt > 1:242359/1‑71 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCAc > 1:1281247/1‑37 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGAt > 1:1481032/1‑52 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGAt > 1:585989/1‑52 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCttt < 1:1203453/70‑1 (MQ=255) cAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCa < 1:113390/68‑1 (MQ=255) aGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCa < 1:1527180/71‑1 (MQ=255) ttGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCg > 1:674093/1‑71 (MQ=255) ggctcgCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCAt > 1:581876/1‑69 (MQ=255) ggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCa < 1:370102/71‑1 (MQ=255) gCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGc < 1:1545008/69‑1 (MQ=255) ggAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGAGAGAt > 1:783872/1‑71 (MQ=255) aCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGATCGCCATCAGGCGAGATTc < 1:158188/70‑1 (MQ=255) | CACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTC > W3110S.gb/2462042‑2462171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |