| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,809,581 | G→A | 61.5% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,809,581 | 0 | G | A | 61.5% | 48.2 / 72.9 | 65 | G52G (GGG→GGA) | mprA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (17/8); new base A (14/26); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATT > W3110S.gb/2809515‑2809641 | tCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGTGAtt > 1:698128/1‑70 (MQ=255) gTCTTTGCATGCACGTGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTa < 1:1469274/69‑1 (MQ=255) gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTa < 1:238057/69‑1 (MQ=255) gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTa < 1:980202/69‑1 (MQ=255) tCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACg < 1:544714/71‑1 (MQ=255) tCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACg < 1:146899/71‑1 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:1676520/1‑68 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:1199793/65‑1 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc > 1:737422/1‑68 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACgag < 1:1701434/69‑1 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:1248719/68‑1 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:1635291/68‑1 (MQ=255) gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACg > 1:656540/1‑71 (MQ=255) catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACg < 1:1062192/70‑1 (MQ=255) tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg < 1:372133/71‑1 (MQ=255) tgcatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt > 1:1190460/3‑71 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCATTAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt < 1:66834/71‑1 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:419955/1‑70 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt < 1:131333/71‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTAt > 1:1364627/1‑71 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTAt < 1:164831/71‑1 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt < 1:549978/68‑1 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:1733953/53‑1 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATgg > 1:1241007/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:1328703/1‑71 (MQ=255) aaaGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt > 1:726271/1‑60 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt < 1:598178/71‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt < 1:1247631/70‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:1379612/71‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:323685/71‑1 (MQ=255) agcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt > 1:1585670/1‑70 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:128641/43‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt < 1:1579601/57‑1 (MQ=255) ctgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc > 1:484227/1‑71 (MQ=255) ctgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:640873/42‑1 (MQ=255) gctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa < 1:615330/38‑1 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag > 1:157123/1‑44 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:325947/69‑1 (MQ=255) agaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg > 1:1084572/1‑71 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc > 1:937809/1‑37 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc > 1:560184/1‑37 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa < 1:1418037/62‑1 (MQ=255) aCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:698932/71‑1 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGt > 1:1653503/1‑53 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:160440/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:1081647/1‑70 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:112165/1‑70 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:534582/1‑70 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACgtt < 1:1239716/36‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:867940/1‑70 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTTGAGTCTCAGGAAAAc > 1:1451802/1‑71 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1405073/1‑39 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGt > 1:425140/1‑45 (MQ=255) aTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1679419/1‑37 (MQ=255) aTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1359770/1‑37 (MQ=255) tGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1664246/1‑36 (MQ=255) tGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAtt < 1:1259498/47‑1 (MQ=255) tGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAtt < 1:1136439/47‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:736029/1‑60 (MQ=255) gAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTa > 1:574683/1‑71 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt < 1:608622/71‑1 (MQ=255) aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAtt < 1:1277322/71‑1 (MQ=255) cAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcaca < 1:478699/61‑1 (MQ=255) aGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcac > 1:1739801/1‑59 (MQ=255) aGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCACAGGAAAACcac > 1:974305/1‑59 (MQ=255) gggaATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:656408/40‑1 (MQ=255) | TCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATT > W3110S.gb/2809515‑2809641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |