| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,966,215 | T→G | 56.6% | K292N (AAA→AAC) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,966,215 | 0 | T | G | 56.6% | 23.8 / 18.9 | 23 | K292N (AAA→AAC) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (3/7); new base G (2/11); total (5/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTT > W3110S.gb/2966148‑2966272 | cTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt > 1:1125176/1‑70 (MQ=255) gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt > 1:978558/1‑67 (MQ=255) aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTg < 1:1310400/67‑1 (MQ=255) aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGAt < 1:24938/71‑1 (MQ=255) tcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACt < 1:1146289/71‑1 (MQ=255) caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACt > 1:1531407/1‑70 (MQ=255) gATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGAt < 1:993779/57‑1 (MQ=255) gATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAgc < 1:766522/71‑1 (MQ=255) aTGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAgcg < 1:1524232/71‑1 (MQ=255) aTCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGt < 1:665122/71‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTa < 1:31182/49‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCa < 1:236233/64‑1 (MQ=255) ccAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaaa < 1:1606418/70‑1 (MQ=255) ccAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaaa < 1:172820/70‑1 (MQ=255) gcACGTAAATCGCCAGCCCGCTCTTTGAGATATTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttttt > 1:1067226/1‑70 (MQ=255) gcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc < 1:443924/71‑1 (MQ=255) cACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:1600622/57‑1 (MQ=255) cACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:1699849/57‑1 (MQ=255) cACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCa < 1:1494214/71‑1 (MQ=255) tAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACtt < 1:1310817/37‑1 (MQ=255) aaaTCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGAc > 1:1245273/1‑71 (MQ=255) tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcg < 1:530400/37‑1 (MQ=255) tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACt > 1:507237/1‑46 (MQ=255) tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACt > 1:877564/1‑46 (MQ=255) gCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGtt < 1:355168/70‑1 (MQ=255) | CTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTT > W3110S.gb/2966148‑2966272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |