| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,001,511 | T→C | 54.8% | I81T (ATA→ACA) | xdhB → | xanthine dehydrogenase, FAD‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,001,511 | 0 | T | C | 54.8% | 8.0 / 38.5 | 31 | I81T (ATA→ACA) | xdhB | xanthine dehydrogenase, FAD‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (6/8); new base C (14/3); total (20/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGA > W3110S.gb/3001446‑3001575 | gggAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATag > 1:279177/1‑68 (MQ=255)gggAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACag > 1:1206779/1‑68 (MQ=255)gggAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACag > 1:1646986/1‑68 (MQ=255) gAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACag > 1:906049/1‑66 (MQ=255) aTTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATcc > 1:1207993/1‑71 (MQ=255) tACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaa < 1:14478/64‑1 (MQ=255) gAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACg > 1:443289/1‑71 (MQ=255) gAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACACAGCTAACAGAAGATCCTATAACTc > 1:86486/1‑67 (MQ=255) aGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCACCGTc < 1:656822/71‑1 (MQ=255) aGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGt > 1:1300656/1‑70 (MQ=255) ggCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCta > 1:1304513/1‑55 (MQ=255) gCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCAtctc > 1:894995/1‑71 (MQ=255) cTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCaa > 1:719005/1‑61 (MQ=255) cTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTc < 1:1007169/65‑1 (MQ=255) cTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTcc > 1:1719970/1‑71 (MQ=255) cTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTcc < 1:694433/71‑1 (MQ=255) cGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCg > 1:824835/1‑71 (MQ=255) cTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc > 1:1354927/1‑71 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt < 1:1042954/70‑1 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGAt < 1:474218/38‑1 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGAt < 1:755697/38‑1 (MQ=255) ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCta > 1:959861/1‑40 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGcc < 1:1066178/71‑1 (MQ=255) aTTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCa < 1:1746668/71‑1 (MQ=255) aTTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCa < 1:1461790/71‑1 (MQ=255) tttACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCAt > 1:385896/1‑71 (MQ=255) tACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTg > 1:713198/1‑71 (MQ=255) cccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGc < 1:1443917/70‑1 (MQ=255) aGCTAATAGAAGATCCTATAACTCGACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGa > 1:1623097/1‑71 (MQ=255) gCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTg > 1:466002/1‑65 (MQ=255) gCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTg > 1:307489/1‑65 (MQ=255) | GGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGA > W3110S.gb/3001446‑3001575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |