| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,054,203 | G→A | 22.2% | intergenic (‑103/‑65) | ygfB ← / → zapA | hypothetical protein/protein that localizes to the cytokinetic ring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,054,203 | 0 | G | A | 22.2% | 52.0 / 11.1 | 27 | intergenic (‑103/‑65) | ygfB/zapA | hypothetical protein/protein that localizes to the cytokinetic ring |
| Reads supporting (aligned to +/- strand): ref base G (10/11); new base A (3/3); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
ATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGAAGGTGGCATGT > W3110S.gb/3054134‑3054271 | atatGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAgtgt > 1:954051/1‑71 (MQ=255) atGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCg > 1:317918/1‑71 (MQ=255) cTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGcc < 1:642810/70‑1 (MQ=255) ccACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCt < 1:1431167/70‑1 (MQ=255) tttGGGACCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGc > 1:376240/1‑62 (MQ=255) cccTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCAt < 1:594437/71‑1 (MQ=255) tggtggCCCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATgg > 1:908956/1‑70 (MQ=255) gtggACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTATCGCCCCTTCGCAGACCATGGGTc > 1:1697284/1‑71 (MQ=255) ggACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCt > 1:996197/1‑70 (MQ=255) gACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCGCTTCGCAGACCATGGGTCTa < 1:252219/70‑1 (MQ=255) gACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCg > 1:418393/1‑55 (MQ=255) gACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTATCGCCCCTTCGCAGACCATGGGTCTa > 1:790234/1‑70 (MQ=255) aCCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGa < 1:430525/58‑1 (MQ=255) aCCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGa < 1:759850/58‑1 (MQ=255) cAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGAcc > 1:742658/1‑58 (MQ=255) tctcTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAgcgc > 1:131886/1‑69 (MQ=255) tcttctCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAg > 1:1502692/1‑54 (MQ=255) tcttctCATCAGGGTAGCTATAGTATCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCa < 1:1197670/63‑1 (MQ=255) cttctCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTc > 1:823743/1‑70 (MQ=255) ttctCATCAGGGTAGCTATAGTATCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCa > 1:978217/1‑70 (MQ=255) ctcATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATgg > 1:1746161/1‑42 (MQ=255) aGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAgg < 1:1512330/71‑1 (MQ=255) gggTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGa < 1:843317/71‑1 (MQ=255) gggTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGa < 1:877110/71‑1 (MQ=255) gggTAGCTATAGTATCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGa < 1:76910/71‑1 (MQ=255) ggTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGaa < 1:1748771/71‑1 (MQ=255) gCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGAAGGTg < 1:346840/71‑1 (MQ=255) taGTATCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGAAGGTGGCAt < 1:1671096/71‑1 (MQ=255) gtgtCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGAAGGTGGCATGt < 1:1590887/71‑1 (MQ=255) | ATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAAGACGAAGGCAGCGCAGTCAATCAGCAGGAAGGTGGCATGT > W3110S.gb/3054134‑3054271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |