| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,139,938 | C→T | 41.7% | R2H (CGT→CAT) | hybD ← | predicted maturation element for hydrogenase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,139,938 | 0 | C | T | 41.7% | 11.0 / 25.6 | 24 | R2H (CGT→CAT) | hybD | predicted maturation element for hydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base C (11/3); new base T (3/7); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
CGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGCCAT > W3110S.gb/3139873‑3140007 | cGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCAt > 1:409842/1‑70 (MQ=255) gACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCAtt < 1:445872/70‑1 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAcc > 1:223020/1‑71 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAcc < 1:458139/71‑1 (MQ=255) aTGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGaa < 1:1080774/71‑1 (MQ=255) aTGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGATCCTTCACTGa > 1:1699688/1‑70 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt > 1:1383915/1‑71 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGaa > 1:944266/1‑51 (MQ=255) aaaaTATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt > 1:1318251/1‑69 (MQ=255) aaaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt > 1:277233/1‑69 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATATGCATTGCAGAACCTTCACTGAAACCACTTCGTTGCCGTCAg > 1:1365637/1‑71 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAg < 1:1133730/71‑1 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGtt > 1:4097/1‑63 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGt > 1:709441/1‑62 (MQ=255) tatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCAt < 1:988482/71‑1 (MQ=255) ttGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATcc < 1:399313/71‑1 (MQ=255) cGACCCCTAAGACTAAAATATGCATTACAGAACCtt < 1:314851/36‑1 (MQ=255) cGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACt < 1:927723/70‑1 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:1658795/1‑71 (MQ=255) aaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt > 1:521001/1‑61 (MQ=255) aaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt > 1:952258/1‑61 (MQ=255) actACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGc < 1:344586/63‑1 (MQ=255) tACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGCCa < 1:24073/71‑1 (MQ=255) aCGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGCCAt > 1:1368434/1‑71 (MQ=255) | CGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGCCAT > W3110S.gb/3139873‑3140007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |