| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,213,568 | C→T | 52.9% | intergenic (+24/+55) | rpoD → / ← ygjF | RNA polymerase, sigma 70 (sigma D) factor/G/U mismatch‑specific DNA glycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,213,568 | 0 | C | T | 52.9% | ‑5.1 / 21.3 | 17 | intergenic (+24/+55) | rpoD/ygjF | RNA polymerase, sigma 70 (sigma D) factor/G/U mismatch‑specific DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base T (4/5); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
CCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCAC > W3110S.gb/3213508‑3213637 | ccGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCg > 1:184531/1‑71 (MQ=255) cTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCgg < 1:1060672/67‑1 (MQ=255) cTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCAc > 1:482220/1‑70 (MQ=255) gcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCctc > 1:1507895/1‑69 (MQ=255) gCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGcaca < 1:842535/70‑1 (MQ=255) gACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCAcc > 1:1640759/1‑71 (MQ=255) gATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCttt < 1:651420/71‑1 (MQ=255) ttAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACaa < 1:1335181/58‑1 (MQ=255) tAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCTGTGGCGt < 1:241469/71‑1 (MQ=255) gCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGtttt < 1:1113823/71‑1 (MQ=255) cAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGc > 1:1309481/1‑71 (MQ=255) gCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGtttt > 1:1634285/1‑61 (MQ=255) cGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAAc > 1:663253/1‑38 (MQ=255) cGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAAc > 1:109173/1‑38 (MQ=255) cGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCAc < 1:1294459/71‑1 (MQ=255) ttATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTc < 1:551632/47‑1 (MQ=255) aCGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGccc > 1:1476049/1‑69 (MQ=255) | CCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCAC > W3110S.gb/3213508‑3213637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |