| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,220,824 | C→A | 33.3% | P235T (CCG→ACG) | ebgR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,220,824 | 0 | C | A | 33.3% | 21.3 / 6.6 | 15 | P235T (CCG→ACG) | ebgR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (6/4); new base A (2/3); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
TGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCC > W3110S.gb/3220755‑3220893 | tGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATcc > 1:339653/1‑71 (MQ=255) gggTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGAtt > 1:492950/1‑71 (MQ=255) tATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCAt > 1:1069853/1‑71 (MQ=255) cTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTc > 1:1465962/1‑56 (MQ=255) gCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtt > 1:1447422/1‑44 (MQ=255) aaaaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg < 1:685762/71‑1 (MQ=255) aaaaCAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg < 1:502474/71‑1 (MQ=255) aaaCAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTcc < 1:356720/51‑1 (MQ=255) aaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTcc < 1:152867/50‑1 (MQ=255) aaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTcc < 1:748160/50‑1 (MQ=255) cAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttgt > 1:240621/1‑41 (MQ=255) cAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACt > 1:834364/1‑71 (MQ=255) aaaTGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgtt < 1:1020252/41‑1 (MQ=255) cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAtt > 1:1638385/1‑71 (MQ=255) aGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc < 1:975070/71‑1 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGcc > 1:1551153/1‑71 (MQ=255) ccGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGaa > 1:1720965/1‑63 (MQ=255) | TGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCC > W3110S.gb/3220755‑3220893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |