Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,220,824 C→A 33.3% P235T (CCG→ACG)  ebgR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,220,8240CA33.3% 21.3 / 6.6 15P235T (CCG→ACG) ebgRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (6/4);  new base A (2/3);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCC  >  W3110S.gb/3220755‑3220893
                                                                     |                                                                     
tGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATcc                                                                      >  1:339653/1‑71 (MQ=255)
                          gggTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGAtt                                            >  1:492950/1‑71 (MQ=255)
                              tATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCAt                                        >  1:1069853/1‑71 (MQ=255)
                                    cTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTc                                                 >  1:1465962/1‑56 (MQ=255)
                                       gCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtt                                                          >  1:1447422/1‑44 (MQ=255)
                                         aaaaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg                             <  1:685762/71‑1 (MQ=255)
                                         aaaaCAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg                             <  1:502474/71‑1 (MQ=255)
                                          aaaCAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTcc                                                <  1:356720/51‑1 (MQ=255)
                                           aaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTcc                                                <  1:152867/50‑1 (MQ=255)
                                           aaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTcc                                                <  1:748160/50‑1 (MQ=255)
                                             cAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgtttgt                                                       >  1:240621/1‑41 (MQ=255)
                                             cAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACt                         >  1:834364/1‑71 (MQ=255)
                                              aaaTGCTGGCGCGGGAAGACTATACGAAGGCACtgtttgtt                                                      <  1:1020252/41‑1 (MQ=255)
                                                       cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAtt               >  1:1638385/1‑71 (MQ=255)
                                                              aGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc        <  1:975070/71‑1 (MQ=255)
                                                                    tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGcc  >  1:1551153/1‑71 (MQ=255)
                                                                     ccGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGaa         >  1:1720965/1‑63 (MQ=255)
                                                                     |                                                                     
TGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCC  >  W3110S.gb/3220755‑3220893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: