| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,224,816 | C→T | 43.3% | H147Y (CAT→TAT) | ebgC → | cryptic beta‑D‑galactosidase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,224,816 | 0 | C | T | 43.3% | 10.5 / 34.6 | 30 | H147Y (CAT→TAT) | ebgC | cryptic beta‑D‑galactosidase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (9/8); new base T (8/5); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAT > W3110S.gb/3224749‑3224884 | tCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTat < 1:22117/70‑1 (MQ=255) ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTataacaaa > 1:717292/1‑71 (MQ=255) ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaa > 1:643945/1‑70 (MQ=255) aTCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaat < 1:1363507/70‑1 (MQ=255) tCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTataacaaat < 1:1025480/69‑1 (MQ=255) gCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataa > 1:928642/1‑61 (MQ=255) gCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataa > 1:1216516/1‑61 (MQ=255) aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg > 1:55054/1‑71 (MQ=255) tCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:1274419/57‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTa > 1:1755521/1‑58 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAgg < 1:602569/71‑1 (MQ=255) aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCaaaa > 1:1635200/1‑68 (MQ=255) aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAg > 1:838618/1‑69 (MQ=255) aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGa < 1:1594071/71‑1 (MQ=255) aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTataacaaa > 1:1485118/1‑47 (MQ=255) aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataacaa > 1:1724762/1‑53 (MQ=255) aGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGt < 1:1026254/71‑1 (MQ=255) aGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGt < 1:740488/71‑1 (MQ=255) gTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg < 1:1189683/70‑1 (MQ=255) tctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg > 1:68211/1‑68 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg < 1:1602598/51‑1 (MQ=255) gTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGc > 1:1065863/1‑71 (MQ=255) ccATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCa > 1:330783/1‑63 (MQ=255) tCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgc < 1:418750/55‑1 (MQ=255) cGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTAc > 1:216061/1‑71 (MQ=255) aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:11821/1‑69 (MQ=255) aTTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGttt < 1:368158/41‑1 (MQ=255) tCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc < 1:1009267/41‑1 (MQ=255) tCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAt > 1:1286358/1‑71 (MQ=255) tCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAt > 1:965388/1‑71 (MQ=255) | TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAT > W3110S.gb/3224749‑3224884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |