| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,233,407 | T→C | 12.9% | G375G (GGA→GGG) | ygjO ← | predicted methyltransferase small domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,233,407 | 0 | T | C | 12.9% | 79.7 / 4.8 | 33 | G375G (GGA→GGG) | ygjO | predicted methyltransferase small domain |
| Reads supporting (aligned to +/- strand): ref base T (19/9); new base C (2/2); total (21/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAA > W3110S.gb/3233351‑3233472 | ccGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGt < 1:1689259/57‑1 (MQ=255)ccGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGGCCCAGCTTCACCGc < 1:172779/71‑1 (MQ=255) ggATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCtt > 1:15499/1‑71 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTc > 1:678396/1‑55 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCttt < 1:1147051/71‑1 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCttt < 1:1568638/71‑1 (MQ=255) ggCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAg < 1:227046/69‑1 (MQ=255) gCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTc < 1:1686596/66‑1 (MQ=255) cGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAg > 1:1173263/1‑67 (MQ=255) gTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACca > 1:691863/1‑71 (MQ=255) ccgcATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCTTCACCGCTTTCAg > 1:1004500/1‑59 (MQ=255) cgcATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCAc > 1:1183002/1‑49 (MQ=255) cgcATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCAc > 1:923406/1‑49 (MQ=255) cgcATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCAc > 1:40267/1‑49 (MQ=255) ccATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCAc > 1:865759/2‑48 (MQ=255) tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGAACCACaa > 1:973119/1‑62 (MQ=255) cGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATtc > 1:87612/1‑71 (MQ=255) cAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCttt < 1:1197404/46‑1 (MQ=255) aCTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCa > 1:1346693/1‑42 (MQ=255) cccAATCTTACCGACGACGCCCCAGCTTCACCGCTTTc < 1:500582/38‑1 (MQ=255) ccAATCTTACCGACGACGCCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtgg > 1:931164/1‑71 (MQ=255) tCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATa > 1:1344350/1‑63 (MQ=255) cTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAg > 1:1186458/1‑71 (MQ=255) ttACCGACGACGTCCCAGCTTCACCGCTTTCAGTACcaca > 1:1084070/1‑40 (MQ=255) ttACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGt > 1:669530/1‑71 (MQ=255) ttACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGt > 1:175961/1‑71 (MQ=255) aCCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAg < 1:740531/68‑1 (MQ=255) aCCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGt < 1:39170/69‑1 (MQ=255) aCCGACGACGCCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGTAg < 1:662485/68‑1 (MQ=255) gacgacGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGcc > 1:946862/1‑70 (MQ=255) cgacgTCCCAGCTTCACCGCTTTCAGTACCACAAAttt > 1:211547/1‑38 (MQ=255) cgacgTCCCAGCTTCACCGCTTTCAGTACCACAAAttt > 1:896032/1‑38 (MQ=255) cgacgTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGaa < 1:1435638/71‑1 (MQ=255) acgTCCCAGCTTCAGCGCTTTGAGTACCACAAATTTATTATTCGTCGCAATAgtgg < 1:1696879/56‑1 (MQ=255) | CCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAA > W3110S.gb/3233351‑3233472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |