| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,244,017 | G→A | 46.9% | Q86Q (CAG→CAA) | exuT → | hexuronate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,244,017 | 0 | G | A | 46.9% | 2.6 / 42.2 | 32 | Q86Q (CAG→CAA) | exuT | hexuronate transporter |
| Reads supporting (aligned to +/- strand): ref base G (12/5); new base A (8/7); total (20/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
TGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTG > W3110S.gb/3243950‑3244086 | tGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTAt > 1:394978/1‑71 (MQ=255) gcgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCt < 1:1724248/71‑1 (MQ=255) tAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAg < 1:1397450/61‑1 (MQ=255) aaCACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTatat > 1:1499628/1‑71 (MQ=255) gtgGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTc > 1:436179/1‑59 (MQ=255) gtgGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTc > 1:1585095/1‑59 (MQ=255) gcggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAg < 1:1695266/70‑1 (MQ=255) cggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGcc > 1:1247437/1‑71 (MQ=255) cggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGcc > 1:1268325/1‑71 (MQ=255) cTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTct > 1:125195/1‑71 (MQ=255) tGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTAt < 1:24801/46‑1 (MQ=255) gcgcCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATatcatc > 1:1057756/1‑57 (MQ=255) aaCTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCtt < 1:299207/71‑1 (MQ=255) tGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg > 1:1382984/1‑71 (MQ=255) tGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg > 1:459229/1‑71 (MQ=255) tGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc < 1:513943/71‑1 (MQ=255) aaaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTct > 1:787290/1‑46 (MQ=255) aaaCATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTtata > 1:1133496/1‑56 (MQ=255) aaaCATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg > 1:1126477/1‑71 (MQ=255) aaCATCTCCACCCAACAGTATTCCTATATCATCGCa > 1:1576487/1‑36 (MQ=255) aaCATCTCCACCCAACAGTATTCCTATATCATCGCa > 1:395049/1‑36 (MQ=255) aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg > 1:423131/1‑69 (MQ=255) cATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAg < 1:69305/71‑1 (MQ=255) aTCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGc < 1:1231689/71‑1 (MQ=255) tctcCACCCAACAATATTCCTATATCATCGCAGCCTa < 1:506732/37‑1 (MQ=255) tctcCACCCAACAATATTCCTATATCATCGCAGCCTa < 1:855836/37‑1 (MQ=255) cccAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTAt > 1:1313283/1‑71 (MQ=255) ccAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATg < 1:1450592/71‑1 (MQ=255) ccAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATg < 1:698483/71‑1 (MQ=255) caacaaTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATg > 1:1292393/1‑70 (MQ=255) cAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGt < 1:1763400/71‑1 (MQ=255) aaCAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTa > 1:1673777/1‑71 (MQ=255) aGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCaa > 1:1073606/1‑50 (MQ=255) aGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCTGTAGCAGGTTATGTACTg > 1:102010/1‑71 (MQ=255) | TGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTG > W3110S.gb/3243950‑3244086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |