| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,294,438 | T→C | 55.6% | L395S (TTG→TCG) | yraM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,294,438 | 0 | T | C | 55.6% | 2.0 / 19.9 | 18 | L395S (TTG→TCG) | yraM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (4/4); new base C (3/7); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGC > W3110S.gb/3294374‑3294502 | cAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGaa < 1:1681129/71‑1 (MQ=255) ggTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa > 1:609496/1‑69 (MQ=255) ttCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGc < 1:1302181/71‑1 (MQ=255) aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTc > 1:1124433/1‑69 (MQ=255) gAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACg < 1:1391696/71‑1 (MQ=255) gAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACg > 1:955619/1‑71 (MQ=255) tATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTAc < 1:334860/71‑1 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTg < 1:805730/41‑1 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCt < 1:1038074/43‑1 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCt < 1:649353/43‑1 (MQ=255) tcggtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTg < 1:1240757/71‑1 (MQ=255) tcggtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTg < 1:574731/71‑1 (MQ=255) cGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTc > 1:1234816/1‑45 (MQ=255) gAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgag > 1:1352978/1‑70 (MQ=255) aaaaaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgag < 1:1729554/69‑1 (MQ=255) caCGTTGAATAGTTGCTGAAGAGCAACACTCCGCTg < 1:1167961/35‑1 (MQ=39) aaGAGTCGCTGAAGAGCAACACTCCGCTGAACGTa > 1:1407435/1‑35 (MQ=255) aaGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATcgc > 1:478508/1‑71 (MQ=255) | CAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGC > W3110S.gb/3294374‑3294502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |