| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,306,434 | G→A | 27.8% | L428L (CTG→TTG) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,306,434 | 0 | G | A | 27.8% | 25.5 / 8.9 | 18 | L428L (CTG→TTG) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (7/6); new base A (3/2); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
GTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAG > W3110S.gb/3306368‑3306497 | gTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGcc < 1:236803/71‑1 (MQ=255) aCGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:993242/71‑1 (MQ=255) aCGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCa < 1:1629159/71‑1 (MQ=255) tCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCagag > 1:347528/1‑69 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:1516635/1‑71 (MQ=255) gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCga < 1:126971/60‑1 (MQ=255) gATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc > 1:398453/1‑71 (MQ=255) aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTc < 1:1077319/53‑1 (MQ=255) cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt > 1:129351/1‑71 (MQ=255) cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATACAGct > 1:34155/1‑62 (MQ=255) agTACGTTCACCCTGTGCCATTTTCAGCAATGCCGc > 1:736482/1‑36 (MQ=255) ttCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc < 1:1145791/59‑1 (MQ=255) ttCACCCTGTGCCATTTTCAGCAGTGCCGAAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCg < 1:851071/71‑1 (MQ=255) cTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGaa > 1:866953/1‑71 (MQ=255) ccATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCa > 1:1337096/1‑39 (MQ=255) ccATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTg < 1:1488181/71‑1 (MQ=255) ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCt > 1:930800/1‑70 (MQ=255) ttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTTAATTTTGCTcag > 1:1118286/1‑71 (MQ=255) | GTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAG > W3110S.gb/3306368‑3306497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |