| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,332,615 | T→A | 52.7% | intergenic (‑25/+102) | yhbE ← / ← rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,332,615 | 0 | T | A | 52.7% | ‑0.4 / 22.8 | 19 | intergenic (‑25/+102) | yhbE/rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base A (3/7); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAC > W3110S.gb/3332558‑3332676 | cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc > 1:93654/1‑71 (MQ=255) aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:720108/64‑1 (MQ=255) aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCg > 1:629854/1‑60 (MQ=255) aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCg > 1:339406/1‑60 (MQ=255) aGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAc < 1:1745743/71‑1 (MQ=255) atgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCAt < 1:954040/71‑1 (MQ=255) aatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCa < 1:752082/71‑1 (MQ=255) aatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCa < 1:1019318/71‑1 (MQ=255) tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGa > 1:1290017/1‑49 (MQ=255) tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGAtt < 1:173117/51‑1 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCAc > 1:1572306/1‑59 (MQ=255) gctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt < 1:575877/55‑1 (MQ=255) ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt < 1:1134390/54‑1 (MQ=255) ctgctTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGt < 1:1668165/70‑1 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:1662830/38‑1 (MQ=255) aTACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAAt < 1:1706971/71‑1 (MQ=255) ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaaa < 1:1473425/70‑1 (MQ=255) cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGccc > 1:439495/1‑70 (MQ=255) aaGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAc < 1:1142682/71‑1 (MQ=255) | CAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAC > W3110S.gb/3332558‑3332676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |