| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,338,549 | G→A | 45.0% | F185F (TTC→TTT) | yrbE ← | predicted toluene transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,338,549 | 0 | G | A | 45.0% | 1.6 / 22.8 | 20 | F185F (TTC→TTT) | yrbE | predicted toluene transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/5); new base A (5/4); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01 | |||||||||||
CGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCA > W3110S.gb/3338485‑3338610 | cGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAc < 1:1027748/71‑1 (MQ=255) ctTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCagaaga < 1:1301636/65‑1 (MQ=255) aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:95971/71‑1 (MQ=255) aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc < 1:221913/71‑1 (MQ=255) aGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAAt > 1:19376/1‑71 (MQ=255) aGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAAt > 1:1100523/1‑71 (MQ=255) cAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGcc > 1:968569/1‑71 (MQ=255) aCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTcc < 1:439309/70‑1 (MQ=255) gATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCaa > 1:836152/1‑57 (MQ=255) gATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACt > 1:370352/1‑70 (MQ=255) aTCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCaa < 1:1595945/56‑1 (MQ=255) ccATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACg > 1:467336/1‑71 (MQ=255) cGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGc > 1:1297018/1‑67 (MQ=255) aGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:1400625/43‑1 (MQ=255) ggCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:1007005/36‑1 (MQ=255) ggCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:318965/36‑1 (MQ=255) ggCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGc > 1:979666/1‑71 (MQ=255) cATTTTGCATTGCCGACCAGAAGAACCCGCTATCaa > 1:217818/1‑36 (MQ=255) cATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc < 1:440457/51‑1 (MQ=255) aTTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATc > 1:1196032/1‑69 (MQ=255) tGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 1:584967/1‑71 (MQ=255) | CGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCA > W3110S.gb/3338485‑3338610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |