| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,363,041 | T→C | 15.4% | L127S (TTA→TCA) | yhcD → | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,363,041 | 0 | T | C | 15.4% | 94.8 / 11.0 | 39 | L127S (TTA→TCA) | yhcD | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (18/15); new base C (3/3); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACA > W3110S.gb/3362978‑3363093 | gTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGatat > 1:457077/1‑70 (MQ=255)gTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAAACAGGAAGCGTTAGata > 1:386307/1‑69 (MQ=255) aCTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGa > 1:1678407/1‑61 (MQ=255) cTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTa < 1:652063/58‑1 (MQ=255) tatataTTGTCCTACCCGGGTACCACAACCACACCATTACCTAACCAGGAAGCGTTAGata > 1:1016847/1‑61 (MQ=255) ttGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcacca > 1:517960/1‑69 (MQ=255) cccGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTCAGATATTATCGTTTCACCACAGGCGAt < 1:955080/69‑1 (MQ=255) ccGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCAt < 1:1440399/71‑1 (MQ=255) cGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTCAGAtattat > 1:689168/1‑49 (MQ=255) tACCACAATCACACCATTACCTGACCAGGAAGCGTTAGATATTATCGTTTcaccac < 1:832208/56‑1 (MQ=255) tACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGt < 1:1377067/48‑1 (MQ=255) aCCACAATCACACCATTACCTAACCAGGAAGCTTTAGATATTATCGTTTcac < 1:1744906/52‑1 (MQ=255) aCCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcac < 1:1266378/52‑1 (MQ=255) aCCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcac < 1:1447275/52‑1 (MQ=255) ccACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcacc > 1:1047066/1‑52 (MQ=255) acaATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGtt > 1:1542252/1‑45 (MQ=255) acaATCACACCATTACCTAACCAGGAAGCGTCAGATATTATCGTTTCACCACAGGCGATCATTCCCATAgg > 1:1101037/1‑71 (MQ=255) aaTCACACCATTACCTAACCAGGAAGCGTCAGATATTATCGTTTCACCACAGGCGATCATTCCCATAg < 1:1070812/68‑1 (MQ=255) tCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTg > 1:779022/1‑71 (MQ=255) cacCATTACCTAACCAGGAAGCGTTAGATATTATCGt > 1:37640/1‑37 (MQ=255) cacCATTACCTAACCAGGAAGCGTTAGATATTATCGt > 1:447286/1‑37 (MQ=255) cacCATTACCTAACCAGGAAGCGTTAGATATTATCGt > 1:428049/1‑37 (MQ=255) cacCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAt < 1:1051186/71‑1 (MQ=255) acCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAtc > 1:959914/1‑71 (MQ=255) ccATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAtct < 1:200915/71‑1 (MQ=255) ccATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAtct < 1:424534/71‑1 (MQ=255) ccATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAtct < 1:1592990/71‑1 (MQ=255) cATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCa < 1:896299/57‑1 (MQ=255) tACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCAtt > 1:384712/1‑50 (MQ=255) tACCTAACCAGGAAGCGTTAGATATTATCGTTTCACAACAGGc > 1:777181/1‑43 (MQ=255) aCCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCa < 1:506807/47‑1 (MQ=255) aCCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCa < 1:1636220/47‑1 (MQ=255) aCCTAACCAGGAAGCGTCAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTcaca > 1:977263/1‑70 (MQ=255) cTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCAtt > 1:1388816/1‑47 (MQ=255) cTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTcaca < 1:467509/68‑1 (MQ=255) cTAACCAGGAAGCGTCAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTcaca < 1:1684913/68‑1 (MQ=255) aaCCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAt > 1:1185653/1‑60 (MQ=255) aaCCAGGAAGCGTTAGATATTATCGTTGCACCACAGGCGATCATTCCCATAGGGTTGGAt > 1:1694853/1‑60 (MQ=255) aGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTca > 1:1263268/1‑60 (MQ=255) | GTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACA > W3110S.gb/3362978‑3363093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |