| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,459,280 | T→C | 60.0% | E15G (GAA→GGA) | secE ← | preprotein translocase membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,459,280 | 0 | T | C | 60.0% | 7.0 / 11.9 | 15 | E15G (GAA→GGA) | secE | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base C (3/6); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTA > W3110S.gb/3459211‑3459348 | tCGCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCtt < 1:1176735/70‑1 (MQ=255) gcgATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTc > 1:1266364/1‑69 (MQ=255) cGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCt > 1:549290/1‑71 (MQ=255) aCAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTg < 1:991373/71‑1 (MQ=255) cAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa < 1:755999/71‑1 (MQ=255) gCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTc < 1:640258/71‑1 (MQ=255) caccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCgt < 1:1505422/71‑2 (MQ=255) accaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCgg > 1:671958/1‑70 (MQ=255) ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAgg < 1:1316253/41‑1 (MQ=255) ccaccaCAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa < 1:304394/69‑1 (MQ=255) ccacAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaac > 1:932868/1‑71 (MQ=255) ccacAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaac > 1:37054/1‑71 (MQ=255) ccacAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaac > 1:76225/1‑71 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCgg < 1:583612/51‑1 (MQ=255) cGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCt < 1:899208/71‑1 (MQ=255) aCTTCATCGCTTCCAGGGCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa > 1:1095321/1‑62 (MQ=255) ttCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTa > 1:1079079/1‑70 (MQ=255) | TCGCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTTTTCTA > W3110S.gb/3459211‑3459348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |