| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,532,412 | T→C | 40.0% | M236T (ATG→ACG) | cpxA → | sensory histidine kinase in two‑component regulatory system with CpxR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,532,412 | 0 | T | C | 40.0% | 4.5 / 13.0 | 15 | M236T (ATG→ACG) | cpxA | sensory histidine kinase in two‑component regulatory system with CpxR |
| Reads supporting (aligned to +/- strand): ref base T (6/3); new base C (3/3); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCAACTG > W3110S.gb/3532358‑3532482 | tCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGt > 1:1639641/1‑71 (MQ=255) ccTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCatgat > 1:1208448/1‑54 (MQ=255) cTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCt < 1:1376507/71‑1 (MQ=255) cTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCagcagc < 1:168053/67‑1 (MQ=255) cTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCagcagc < 1:1733258/67‑1 (MQ=255) gCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCt > 1:519316/1‑71 (MQ=255) gCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCt > 1:55780/1‑71 (MQ=255) cAGGTGCCAGTTTTAACCAGATTGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCt < 1:954194/71‑1 (MQ=255) aGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGTCCTCTCAGCAGCGTCTGCTTTCTGATAtctc > 1:1289214/1‑71 (MQ=255) tttAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCtctc > 1:1459029/1‑43 (MQ=255) tttAACCAGATGGTCACCGCGCTGGAGCGCATGACTACCtctc > 1:174769/1‑43 (MQ=38) ttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCt > 1:1215962/1‑55 (MQ=255) gATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTgc > 1:1177490/1‑71 (MQ=255) cgcTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGat < 1:1197089/44‑1 (MQ=255) cgcTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGat < 1:395264/44‑1 (MQ=255) gcgcATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTc > 1:1576587/1‑71 (MQ=255) tgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCAACTg < 1:1630223/71‑1 (MQ=255) | TCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCAACTG > W3110S.gb/3532358‑3532482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |