| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,584,576 | G→A | 68.7% | P21S (CCA→TCA) | hemN ← | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,584,576 | 0 | G | A | 68.7% | 14.2 / 10.1 | 16 | P21S (CCA→TCA) | hemN | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base A (7/4); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
TTGTAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCT > W3110S.gb/3584508‑3584639 | ttGTAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGAcc > 1:1248761/1‑71 (MQ=255) tCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGATACGAGGTGTATCGTGACCCGGAATAGTTATAttt > 1:862400/1‑71 (MQ=255) cGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATAttt < 1:219769/70‑1 (MQ=255) tcttctGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCa < 1:1143586/70‑1 (MQ=255) tcttctGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCa < 1:1247607/70‑1 (MQ=255) ctGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCt > 1:1730428/1‑60 (MQ=255) cAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCa > 1:1402977/1‑56 (MQ=255) cAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCa > 1:1490060/1‑56 (MQ=255) gcgGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGAt > 1:1254180/1‑71 (MQ=255) gggTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCt < 1:1090039/70‑1 (MQ=255) aCGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCa > 1:1626280/1‑42 (MQ=255) gAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGa > 1:542952/1‑48 (MQ=255) gAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGa < 1:1149017/71‑1 (MQ=255) tATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCg < 1:196880/51‑1 (MQ=255) aTCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCt > 1:381504/1‑70 (MQ=255) gTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACa > 1:732155/1‑59 (MQ=255) | TTGTAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCT > W3110S.gb/3584508‑3584639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |