| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,615,349 | A→G | 23.8% | P369P (CCT→CCC) | ubiB ← | 2‑octaprenylphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,615,349 | 0 | A | G | 23.8% | 34.0 / 8.5 | 21 | P369P (CCT→CCC) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base A (10/6); new base G (2/3); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
ATTAAACAGATTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTATCTGGTGGCACCCAGC > W3110S.gb/3615289‑3615413 | aTTAAACAGATTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGa > 1:653029/1‑71 (MQ=255) ttAAACAGATTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGAc > 1:1271875/1‑71 (MQ=255) tAAACAGATTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATGGGTTCACAGa > 1:940421/1‑69 (MQ=255) aaCAGATTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCg < 1:489581/70‑1 (MQ=255) cAGATTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTAc > 1:150339/1‑71 (MQ=255) aTTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGaa > 1:380096/1‑71 (MQ=255) aCAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTca > 1:1694138/1‑53 (MQ=255) cATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGc > 1:1007571/1‑64 (MQ=255) tCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGa < 1:1369423/68‑1 (MQ=255) tCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGa < 1:747787/68‑1 (MQ=255) cGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCaa < 1:432951/71‑1 (MQ=255) aTTTCGGCCAGCGGTTTCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGtt < 1:990294/71‑1 (MQ=255) tttCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTg < 1:181324/71‑1 (MQ=255) cGGCCAGCGGTTTCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGt < 1:1250504/70‑1 (MQ=255) cAGCGGTTTCTCAAAGATGGGTTCACAGACCGTACGAATgg < 1:655892/41‑1 (MQ=255) cAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTATCtg < 1:1093103/71‑1 (MQ=255) cGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTATCtggtg > 1:578975/1‑71 (MQ=255) tcAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTATCTGGTGGCAcc > 1:580532/1‑69 (MQ=255) aaaGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTATCTGGTGGCACccagc > 1:630858/1‑71 (MQ=255) aaaGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTATCTGGTGGCACccagc > 1:150493/1‑71 (MQ=255) aTAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt > 1:620865/1‑52 (MQ=255) | ATTAAACAGATTTAACAGTACATGTCCAAACGAAATTTCGGCCAGCGGTTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTATCTGGTGGCACCCAGC > W3110S.gb/3615289‑3615413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |